Description :CD45.2 is an alloantigen of CD45, expressed by Ly5.2 bearing mouse strains (e.g., A, AKR,BALB/c, CBA/Ca, CBA/J, C3H/He, C57BL, C57BR, C57L, C58, DBA/1, DBA/2, NZB, SWR, 129).CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kDglycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets.There are multiple isoforms in the mouse that play key roles in TCR and BCR signaltransduction. These isoforms are very specific to the activation and maturation states of thecell as well as specific cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4,TCR, CD22, and Thy-1.
Verified Reactivity :Mouse
Antibody Type :Monoclonal
Host Species :Mouse
Immunogen :B10.S mouse thymocytes and splenocytes
Formulation :Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation :The antibody was purified by affinity chromatography and conjugated with Spark NIR™ 685 underoptimal conditions.
Concentration :0.5 mg/mL
Storage & Handling :The antibody solution should be stored undiluted between 2°C and 8°C, and protected fromprolonged exposure to light. Do not freeze.
Application :FC - Quality tested
Recommended Usage :Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometricanalysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cellsin 100 µL volume. It is recommended that the reagent be titrated for optimal performance for eachapplication.
* Spark NIR™ 685 has a maximum excitation of 665 nm and a maximum emission of 685 nm.