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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
齐源
- 库存:
999
- 靶点:
CD3
- 级别:
CD3 Antibody
- 目录编号:
CD3 Antibody
- 克隆性:
单克隆
- 抗原来源:
大鼠
- 保质期:
CD3 Antibody
- 抗体英文名:
Alexa Fluor® 488 anti-mouse CD3 Antibody
- 抗体名:
CD3 Antibody
- 标记物:
CD3 Antibody
- 宿主:
大鼠
- 适应物种:
小鼠
- 免疫原:
CD3 Antibody
- 亚型:
CD3 Antibody
- 形态:
CD3 Antibody
- 应用范围:
FC, IHC-F, 3D IHC
- 保存条件:
2-8℃
- 浓度:
0.5 mg/mL
- 规格:
25 ug/100 ug
| 规格: | 25 ug | 产品价格: | ¥1700.0 |
|---|---|---|---|
| 规格: | 100 ug | 产品价格: | ¥3900.0 |
Description :CD3, also known as T3, is a member of the Ig superfamily and primarily expressed on T cells,NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 is composedof CD3ε, δ, γ and ζ chains. It forms a TCR complex by associating with TCR α/β or γ/δ chains.CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognitionby binding the peptide/MHC antigen complex
Verified Reactivity :Mouse
Antibody Type :Monoclonal
Host Species :Rat
Immunogen :γδTCR-positive T-T hybridoma D1
Formulation :Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation :The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488under optimal conditions.
Concentration :0.5 mg/mL
Storage & Handling :The antibody solution should be stored undiluted between 2°C and 8°C, and protected fromprolonged exposure to light. Do not freeze.
Application :FC - Quality tested、IHC-F, 3D IHC - Verified
Recommended Usage :Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometricanalysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cellsin 100 µL volume. For immunohistochemical staining on frozen tissue sections, the suggested useof this reagent is 5.0 - 10 µg per mL. For 3D immunohistochemistry on formalin-fixed tissues, aconcentration of 5.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimalperformance for each application.
* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.
Excitation Laser :Blue Laser (488 nm)
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文献和实验1. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
2. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations:
1. Wu J, et al. 2022. Nat Commun. 13:7321. PubMed
2. Suresh R, et al. 2020. J Immunother Cancer. 8:. PubMed
3. Okajima T, et al. 2021. Front Immunol. 12:738041. PubMed
4. Bieber M, et al. 2021. Int J Mol Sci. 22:. PubMed
5. Schneider C, et al. 2018. Cell. 174:271. PubMed
6. Dauer Née Joppe K, et al. 2021. J Neurochem. 159:554. PubMed
7. Datta S, et al. 2014. J Leukoc Biol. 95:853. PubMed
8. Baptista AP et al. 2019. Immunity. 50(5):1188-1201 . PubMed
9. Brandt S, et al. 2020. J Am Soc Nephrol. 31:2589. PubMed
10. Park JJ, et al. 2021. Nat Commun. 12:1222. PubMed
11. Upadhyaya P, et al. 2021. J Immunother Cancer. 9:00. PubMed
12. Chow MT et al. 2019. Immunity. 50(6):1498-1512 . PubMed
常用抗体标记荧光染料的选择 随着免疫荧光技术的不断发展,荧光染料及其标记的抗体偶联物也被广泛的应用于生物学实验中。目前,市场上抗体及蛋白标记的荧光染料主要有CFTM系列(BIOTIUM, USA); Alexa Fluor®系列 (Life technology, USA); DyLight系列;Cy系列; IR Dye系列等等。使用最多的为AlexaFluor®系列和CFTM系列。CFTM系列染料的核心对比AlexaFluor®系列具有以下几点优势:1. 新型罗丹明核心罗丹明染料以优异
分别利用 CD3 与 CD28 的功能学抗体,以及 CD3/CD28 Streptamer® 激活扩增 T 细胞的实验步骤与实验结果。 CD3/CD28抗体法 1、实验步骤: 1.1 抗体包被 用无菌 PBS 将 anti-mouse CD3 抗体(克隆号:145-2C11)稀释至 5μg/ml,稀释后的抗体加入到 24 孔板中,每孔 400μl,4°C 包被过夜。(注:板子在加入抗体之前,先用无菌 PBS 润洗 2-3 遍,避免干孔); 1.2 小鼠脾脏淋巴细胞分离(次日) 1. 将 70μm
技术原理与方法|超级色差校正物镜 PLAPON60XOSC 在脑组织四重免疫荧光分析中的应用
种类型的荧光抗体在单个标本中准确展示荧光标记分子的共定位情况。 PLAPON60XOSC 和 UPLSAPO60XO 的性能比较 2. 超级色差校正物镜的应用-脑组织的四重免疫荧光 图 1. 脑组织的四重免疫荧光。 VIAAT 染色(Alexa Fluor405,蓝色);CB1 染色(Alexa Fluor488,绿色);VGluT3 染色(Cy3,红色);DGLα染色(Alexa Fluor647,白色)。 大麻素合成酶 DGLα(白色)和大麻素受体 CB1(绿色)聚集在小鼠










