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- 齐源
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- 2025年07月14日
- ICFC
- 小鼠
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
齐源
- 库存：
999
- 靶点：
Nanog
- 级别：
Nanog Antibody
- 目录编号：
Nanog Antibody
- 克隆性：
单克隆
- 抗原来源：
小鼠
- 保质期：
Nanog Antibody
- 抗体英文名：
Alexa Fluor® 647 anti-Nanog Antibody
- 抗体名：
Nanog Antibody
- 标记物：
Nanog Antibody
- 宿主：
小鼠
- 适应物种：
人
- 免疫原：
Nanog Antibody
- 亚型：
Nanog Antibody
- 形态：
Nanog Antibody
- 应用范围：
ICFC
- 保存条件：
2-8℃
- 浓度：
0.5 mg/ml
- 规格：
100 ug
Description ：Nanog is a homeodomain-containing transcription factor that is essential for early embryonicdevelopment. It is primarily expressed in pluripotent embryonic stem (ES) cells and cancer celllines, maintaining the undifferentiated status of the cells and is downregulated upondifferentiation. Si-RNA mediated knockdown of Nanog in ES cells induces lineagedifferentiation. Overexpression of Nanog allows for the leukemia inhibitory factor (LIF)-independent self-renewal in undifferentiated ES cells. Nanog functionally works with OCT4 andSOX2 to support pluripotency and self-renewal in ES cells and the expression of Nanog isregulated by OCT4/SOX2 heterodimers. Expression of Nanog has been detected in severaltypes of tumor cells. The expression level of Nanog in cancer cells has been shown to becorrelated with the malignancy of cancers.
Verified Reactivity ：Human
Antibody Type ：Monoclonal
Host Species ：Mouse
Immunogen ：Full length NANOG recombinant protein expressed in E. coli.
Formulation ：Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation ：The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647under optimal conditions.
Concentration ：0.5 mg/ml
Storage & Handling ：The antibody solution should be stored undiluted between 2°C and 8°C, and protected fromprolonged exposure to light. Do not freeze.
Application ：ICFC - Quality tested
Recommended Usage ：Each lot of this antibody is quality control tested by intracellular flow cytometry using our TrueNuclear™ Transcription Factor Staining Protocol. For flow cytometric staining, the suggested useof this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent betitrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Excitation Laser ：Red Laser (633 nm)
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文献和实验1. Petters J, et al. 2020. Stem Cell Research. 72.42222222. PubMed
2. V ölkner C, et al. 2020. Stem Cell Research. 41:101606.. PubMed
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