- ￥6500 - 11000
- 齐源
- 133301
- 国产/进口
- 2025年07月15日
- FC
- Lineage Cocktail with Isotype Ctrl
- 小鼠
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
齐源
- 库存：
999
- 靶点：
Lineage Cocktail with Isotype Ctrl
- 级别：
Lineage Cocktail with Isotype Ctrl
- 目录编号：
Lineage Cocktail with Isotype Ctrl
- 克隆性：
单克隆
- 抗原来源：
Lineage Cocktail with Isotype Ctrl
- 保质期：
Lineage Cocktail with Isotype Ctrl
- 抗体英文名：
anti-mouse Lineage Cocktail with Isotype Ctrl-FITC
- 抗体名：
Lineage Cocktail with Isotype Ctrl
- 标记物：
Lineage Cocktail with Isotype Ctrl
- 宿主：
Lineage Cocktail with Isotype Ctrl
- 适应物种：
小鼠
- 免疫原：
Lineage Cocktail with Isotype Ctrl
- 亚型：
Lineage Cocktail with Isotype Ctrl
- 形态：
Lineage Cocktail with Isotype Ctrl
- 应用范围：
FC
- 保存条件：
2-8℃
- 浓度：
Lineage Cocktail with Isotype Ctrl
- 规格：
25 tests/100 tests
| 规格： | 25 tests | 产品价格： | ￥6500.0 |
|---|---|---|---|
| 规格： | 100 tests | 产品价格： | ￥11000.0 |
Description ：The mouse lineage panel has been designed to react with cells from the major hematopoieticcell lineages, such as T lymphocytes, B lymphocytes, monocytes/macrophages, granulocytes,NK cells, and erythrocytes. The FITC Mouse Lineage Antibody Cocktail is designed for the flowcytometric identification of hematopoietic progenitors in mouse bone marrow. Componentsinclude anti-mouse CD3ε, clone 145-2C11; anti-mouse Ly-6G/Ly-6C, clone RB6-8C5; antimouse CD11b, clone M1/70; anti-mouse CD45R/B220, clone RA3-6B2; anti-mouse TER119/Erythroid cells, clone Ter-119. FITC Mouse Lineage Isotype Control Cocktail containsequivalent concentrations of isotype-matched negative control immunoglobulin.
Materials provided:1. FITC anti-mCD3ε/ FITC anti-mGr-1/ FITC anti-mCD11b/ FITC anti-mCD45R(B220)/FITC anti-mTer-119, 25 tests2. FITC Armenian hamster IgG/ FITC Rat IgG2b/ FITC Rat IgG2a isotype controls, 25 tests
Materials not provided:Cell Staining Buffer (Cat. No. 420201)
Verified Reactivity ：Mouse
Formulation ：This two-reagent set contains FITC conjugated antibodies and isotype controls in optimalconcentration for flow cytometric analysis.
Preparation ：The antibodies are purified by affinity chromatography, and conjugated with FITC under optimalconditions.
Storage & Handling ：The antibody solutions should be stored undiluted between 2°C and 8°C, and protected fromprolonged exposure to light. Do not freeze.
Application ：FC - Quality tested
Recommended Usage ：Each lot of these antibodies is quality control tested by immunofluorescent staining with flowcytometric analysis. For flow cytometric staining, the suggested use of this reagent is 20 µl permillion cells or 20 µl per 100 µl of whole blood. It is recommended that the reagent be titrated foroptimal performance for each application.
Excitation Laser ：Blue Laser (488 nm)
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文献和实验1. Morris SJ, et al. 1997. Development. 124(10):1929-1939
2. Okada S, et al. 1992. Blood180 (12):3044-3050
3. Spangrude GJ, et al. 1988. Science 241 (4861):58-62
4. Spangrude GJ, et al. 1990. Exp Hematol. 18 (8):920-926
5. Tan SL, et al. 2006. J. Immunol. 176:2872. PubMed
6. Ito R, et al. 2012. Exp Hematol. 40:953. PubMed.
Product Citations：
1. Cai Q, et al. 2018. Blood Cells Mol Dis. 71:23. PubMed
2. Xue Y et al. 2017. Developmental cell. 42(4):349-362 . PubMed
3. Chen G, et al. 2014. Cancer Immunol Res. 2:949. PubMed
4. Wu G, et al. 2020. Br J Pharmacol. 177:5063. PubMed
5. Liu J, et al. 2017. Am J Pathol. 10.1016/j.ajpath.2017.02.010. PubMed
6. Capitano ML, et al. 2020. Blood Cells Mol Dis. 102435:84. PubMed
7. Gao S, et al. 2022. Cell Res. 32:38. PubMed
8. Calcinotto A, et al. 2018. Nat Commun. 9:4832. PubMed
9. Zhang Y, et al. 2021. J Immunol Res. 2021:5599439. PubMed
10. Wang X, et al. 2018. Cell Death Dis. 9:578. PubMed
11. Oka Y, et al. 2020. Sci Adv. 6:. PubMed
12. Capitano ML, et al. 2019. J Clin Invest. 130:2555. PubMed
A c-Kit (2B8) FITC-IgG2b, 01904D FITC-IgG2b Isotype control, 11184C Fc RII block (clone 2.4G2), 01241D Media: Heat inactivated FBS (56°C x 30 min) PBS + 2% heat-inactivated
对照应该是APC标记的Mouse IgG1,κ，货号是555751。注意同型对照的成份跟它的名称是相同的.如果是抗体的组合形式是纯化的一抗＋荧光标记的二抗，那么应该选择一抗的同型对照。比如CDw125的纯化抗体，货号是555901，成分是Mouse IgG1,κ。它的同型对照是纯化的Mouse IgG1,κ，货号是555746。它的二抗PE标记的抗小鼠IgG1,货号是550083。那么染色方式如下： 样本管：纯化的CDw25＋PE标记的抗Mouse IgG1＋
Transplantation Models to Characterize the Mechanisms of Stem Cell–Induced Islet Regeneration
(DPBS, no Ca2+ no Mg2+ ; Life Technologies, cat. no. 14190250) containing 5% (v/v) fetal bovine serum (FBS) Desired fluorescently labeled anti‐human antibodies and isotype controls (Table
