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- 文献和实验
- 技术资料
- 供应商：
齐源
- 库存：
999
- 靶点：
CD64
- 级别：
anti-human CD64 Antibody
- 目录编号：
anti-human CD64 Antibody
- 克隆性：
单克隆
- 抗原来源：
小鼠
- 保质期：
anti-human CD64 Antibody
- 抗体英文名：
anti-human CD64 Antibody
- 抗体名：
CD64抗体
- 标记物：
anti-human CD64 Antibody
- 宿主：
小鼠
- 适应物种：
人、猴
- 免疫原：
anti-human CD64 Antibody
- 亚型：
anti-human CD64 Antibody
- 形态：
anti-human CD64 Antibody
- 应用范围：
FC
- 保存条件：
2-8℃
- 浓度：
anti-human CD64 Antibody
- 规格：
25 tests/100 tests
| 规格： | 25 tests | 产品价格： | ￥3800.0 |
|---|---|---|---|
| 规格： | 100 tests | 产品价格： | ￥7500.0 |
Description
CD64 is a 72 kD single chain type I glycoprotein also known as FcγRI and FcR I. CD64 is amember of the immunoglobulin superfamily and is expressed on monocytes/macrophages,dendritic cells, and activated granulocytes. The expression can be upregulated by IFN-γstimulation. CD64 binds IgG immune complex. It plays a role in antigen capture, phagocytosisof IgG/antigen complexes, and antibody-dependent cellular cytotoxicity (ADCC).
Verified Reactivity ：Human, Cynomolgus, Rhesus
Reported Reactivity ：Baboon, Capuchin Monkey, Chimpanzee, Squirrel Monkey
Antibody Type ：Monoclonal
Host Species ：Mouse
Immunogen ：Human rheumatoid synovial fluid cells and fibronectin-purified monocytes.
Formulation ：Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation ：The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™under optimal conditions
Concentration ：Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysis online tool.)
Storage & Handling ：The antibody solution should be stored undiluted between 2°C and 8°C, and protected fromprolonged exposure to light. Do not freeze.
Application ：FC - Quality tested
Recommended Usage ：Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometricanalysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in100 µl staining volume or 5 µl per 100 µl of whole blood.
Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license fromSirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use thepurchased product for research purposes only. This product may not be resold or incorporated inany manner into another product for resale. Any use for therapeutics or diagnostics is strictlyprohibited. This product is covered by U.S. Patent(s), pending patent applications and foreignequivalents.
Excitation Laser ：Violet Laser (405 nm)
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文献和实验1. McMichael A, et al. Eds. 1987. Leucocyte Typing III. Oxford University Press. New York.
2. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. p. 874.
3. Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London.
4. Holl V, et al. 2004. J. Immunol. 173:6274.
5. Hober D, et al. 2002. J. Gen. Virol. 83:2169.
6. Cho HJ, et al. 2007. Physiol Genomics 149:60.
7. van Tits L, et al. 2005. Arterioscler Thromb Vasc Biol. 25:717. PubMed
8. Bruhns P, et al. 2008. Blood 113:3716. PubMed
9. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
10. Carter DL, et al. 1999. Cytometry 37:41. (FC)
11. Dougherty GJ, et al. 1987. Eur. J. Immunol. 17:1453.
12. Blom AB, et al. 2003. Arthritis Rheum. 48(4):1002-14. (IHC)
Product Citations
1. Zhu B, et al. 2021. Immunity. 54(6):1200-1218.e9. PubMed
2. Abdul-Jawad S, et al. 2021. Cancer Cell. 39(2):257-275.e6. PubMed
3. Laing AG, et al. 2020. Nat Med. 26:1623. PubMed
4. Huynh L, et al. 2016. Sci Rep. 6:31959. PubMed
Elabscience|肺泡巨噬细胞研究必看：LPS诱导急性肺炎模型制备与检测全攻略
Violet 421 标记抗小鼠MHC II（I-A/I-E）抗体[M5/114] AN00821Q2 Elab Bright™ Violet 421 标记大鼠IgG2b，κ同型对照抗体[R35-38] E-AB-F1136S Elab Fluor® Red 780 标记抗小鼠CD45抗体[30-F11] E-AB-F1081T Elab Fluor® Violet 610 标记抗小鼠/人/猴CD11b抗体[M1/70] E-AB-F1186C FITC 标记抗小鼠CD64/FcγRI
肿瘤微环境流式结果总是不稳定？你可能忽略了这几个关键细节（二）
与细胞之间的非特异性结合。本文将从 肿瘤微环境（Tumor Microenvironment, TME） 细胞生物学特征出发，解析三类非特异性染色发生机制，并介绍在肿瘤微环境免疫流式检测中进行封闭的实验步骤。 01 Fc 受体高表达引起的非特异性抗体结合（细胞与抗体之间的非特异性结合） TME 内TAM、MDSC、瘤周髓系细胞、间质髓系 DC 等都高度表达 CD16/CD32、CD64 等 Fcγ 受体，远高于外周血正常免疫
Elabscience 光谱流式全流程详解：从入门到实操，一文讲透
。此时可以考虑引入UMAP或T-SNE等降维分析算法，将高维数据压缩映射到二维空间进行可视化和聚类分析。 数据呈现：最后统计各组群细胞比例或荧光强度中位数（MFI），选择合适的图表（如散点图、直方图、热图）呈现最终结果。 图1. 人外周血T细胞16色全光谱分析和T-SNE降维分析结果 表2. 相关流式抗体汇总 检测指标 克隆号 荧光素 货号 CD8 OKT-8 Elab Fluor® Violet 610
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