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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
齐源
- 库存:
999
- 靶点:
CCX-CKR
- 级别:
CCX-CKR (CCRL1) Antibody
- 目录编号:
CCX-CKR (CCRL1) Antibody
- 克隆性:
单克隆
- 抗原来源:
小鼠
- 保质期:
CCX-CKR (CCRL1) Antibody
- 抗体英文名:
Cyanine7 anti-human CCX-CKR (CCRL1) Antibody-PE
- 抗体名:
CCX-CKR (CCRL1) Antibody
- 标记物:
PE
- 宿主:
小鼠
- 适应物种:
人
- 免疫原:
CCX-CKR (CCRL1) Antibody
- 亚型:
CCX-CKR (CCRL1) Antibody
- 形态:
CCX-CKR (CCRL1) Antibody
- 应用范围:
FC
- 保存条件:
2-8℃
- 浓度:
CCX-CKR (CCRL1) Antibody
- 规格:
25 tests/100 tests
| 规格: | 25 tests | 产品价格: | ¥2700.0 |
|---|---|---|---|
| 规格: | 100 tests | 产品价格: | ¥5900.0 |
Description :Human CCX-CKR, also known as CCRL1, is a 40 kD, G-protein-coupled, seventransmembrane receptor. CCRL1 is a decoy receptor for CCL2, CCL8, CCL13, CCL19, CCL21and CCL25 that regulates chemotaxis of lymphocytes and cancer cells. Chemokine binding byCCX-CKR causes its internalization, reducing levels of available chemokine, and thusregulating migration of lymphocytes and cancerous cells. CCX-CKR is expressed by stromalcells of skin-draining lymph nodes, thymic epithelial cells, and immature dendritic cells (DCs). Alow CCX-CKR expression in tumor cells correlates with a poor survival rate of breast cancerpatients.
Verified Reactivity :Human
Antibody Type :Monoclonal
Host Species :Mouse
Immunogen :Full length CCX-CKR cDNA
Formulation :Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation :The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 underoptimal conditions.
Concentration :Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysis online tool.)
Storage & Handling :The antibody solution should be stored undiluted between 2°C and 8°C, and protected fromprolonged exposure to light. Do not freeze.
Application :FC - Quality tested
Recommended Usage :Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometricanalysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser :Blue Laser (488 nm)、Green Laser (532 nm)、Yellow-Green Laser (561 nm)
Application Notes :Additional reported applications for the relevant formats include: immunoprecipitation of CCX-CKRfrom cell lysates , induction of CCX-CKR internalization and immunofluorescence . 13E11recognizes an epitope located within the first 14 amino acids of CCX-CKR.
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文献和实验1. Takatsuka, S. et al. 2011. J. Pharmacol. Toxicol. Methods. 63:250. (IF, IP, Agonist)
5.5, APC等。 常见荧光素波长分布示意图 同型对照的选择: 流式细胞实验和其他抗体相关实验有点不同的就是需要选择同型对照。同型对照,是用于消除由于抗体非特异性结合到细胞表面而产生的背景染色,相当于实验的阴性对照。同型对照选择与标记抗体同种属来源,同亚型,同荧光标记的抗体。比如:抗人的CD3的PE标记的抗体,小鼠的IgG2a。同型
非特异性结合到细胞表面而产生的背景染色,相当于实验的阴性对照。同型对照选择与标记抗体同种属来源,同亚型,同荧光标记的抗体。比如:抗人的CD3的PE标记的抗体,小鼠的IgG2a。同型对照选择PE标记的小鼠的IgG2a。 流式抗体使用注意事项: 1. 建议实验细胞的数量和抗体的比例要适当。细胞过量或抗体过量都可能使实验结果受影响,因此需要优化试验条件。注:不同的流式技术对抗体的需求量有较大差异,例如传统流式细胞术(采用鞘液流系统)需要1×106个细胞为起始上样量,抗体用量为10 μl
对照选择与标记抗体同种属来源,同亚型,同荧光标记的抗体。比如:抗人的CD3的PE标记的抗体,小鼠的IgG2a。同型对照选择PE标记的小鼠的IgG2a。 流式抗体使用注意事项: 1、建议实验细胞的数量和抗体的比例要适当。细胞过量或抗体过量都可能使实验结果受影响,因此需要优化试验条件。注:不同的流式技术对抗体的需求量有较大差异,例如传统流式细胞术 (采用鞘液流系统)需要1×106个细胞为起始上样量,抗体用量为10μl,而微流式细胞术 则只需5×105个细胞,抗体用量减少到5μl。 2、直标的流式抗体










