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文献和实验,and the cell pellets were frozen and stored at –80ºC. Lysis Lysis was accomplished with B-PER reagent supplemented with 1/50 vol 10-mg/ml chicken egg-white lysozyme (stored at –80ºC in aliquots),1/125 vol 5-mg/ml DNaseI (in 20 mM sodium acetate,pH adjusted
【资源】全面归类网址：毕赤酵母表达各种来源蛋白（细菌，真菌，植物，人类等）
[151,152,153, 387]Bovine lysozyme c2S, 550 mg/L, native[147]Bovine mannose 6-phosphate receptorsS, α-MF, 28 mg purify. protein/L517Bovine opsinS*, 0.3 mg/L, PHO1[148]Bovine pancreatic trypsin inhibitor (aprotinin)S, 930 mg/L, α-MF[149]Bovine tissue-type
Raf 蛋白 Ras 结合结构域的简并进化库合成以及利用片段互补法快速筛选二氢叶酸还原酶的快速折叠且稳定的克隆
thermostable chicken lysozyme.Protein. Sci. 4 , 2063-2072. 9. Riddle, D. S. , Santiago, J. V. , Bray-Hall, S. T. , et al. ( 1 997) Functional rapidly folding proteins from simpli-fied amino acid sequences. Nat. Struct. Biol. 4 , 805
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