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文献和实验Oligodeoxyribonucleotides Synthesis: Phosphoramidite Approach
The development of deoxyribonucleoside phosphoramidite derivatives for the synthesis of oligodeoxyribonucleotides was first described by Beaucage and Caruthers ( 1 ) in 1981. The conceptual basis of this methodology emerged as a modification
Oligoribonucleotide Synthesis: The Silyl-Phosphoramidite Method
In 1968, one of us began a quest to develop a general method for the synthesis of RNA sequences. The major problems associated with the chemical assembly of ribonucleotide chains were already well established. RNA sequences are very sensitive
synthetic protocol for a 2?selenothymidine phosphoramidite and its use in preparing Se?derivatized oligonucleotides are described here. The Se?modified phosphoramidite synthesis was achieved by activating a 2?thiothymidine derivative, followed
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