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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
齐源
- 库存:
9999
- 靶点:
CD274
- 级别:
mouse CD274 (B7-H1, PD-L1) Antibody
- 目录编号:
mouse CD274 (B7-H1, PD-L1) Antibody
- 克隆性:
单克隆
- 抗原来源:
大鼠
- 保质期:
mouse CD274 (B7-H1, PD-L1) Antibody
- 抗体英文名:
Cyanine5 anti-mouse CD274 (B7-H1, PD-L1) Antibody-PE
- 抗体名:
CD274(B7-H1,PD-L1)抗体
- 标记物:
PE
- 宿主:
大鼠
- 适应物种:
小鼠
- 免疫原:
mouse CD274 (B7-H1, PD-L1) Antibody
- 亚型:
mouse CD274 (B7-H1, PD-L1) Antibody
- 形态:
mouse CD274 (B7-H1, PD-L1) Antibody
- 应用范围:
FC
- 保存条件:
2-8℃
- 浓度:
0.2 mg/mL
- 规格:
25 ug/100 ug
| 规格: | 25 ug | 产品价格: | ¥2600.0 |
|---|---|---|---|
| 规格: | 100 ug | 产品价格: | ¥7400.0 |
Description :CD274, also known as B7-H1 or programmed death ligand 1 (PD-L1), is a 40 kD type Itransmembrane protein and a member of the B7 family within the immunoglobulin receptorsuperfamily. It is expressed on T cells, B cells, NK cells, dendritic cells, IFN-γ activatedendothelial cells, and monocytes. B7-H1 is one of the ligands of PD-1. The interaction of B7-H1with PD-1 plays an important role in the inhibition of T cell responses. Other studies haveshown that B7-H1 is able to costimulate T cell growth and cytokine production. CD274 isinvolved in costimulation essential for T cell proliferation and production of IL-10 and IFN-γ, inan IL-2-dependent and a PD-1-independent manner. Its interaction with PD-1 inhibits T cellproliferation and cytokine production.
Verified Reactivity :Mouse
Antibody Type :Monoclonal
Host Species :Rat
Formulation :Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation :The antibody was purified by affinity chromatography and conjugated with PE/Cyanine5 underoptimal conditions.
Concentration :0.2 mg/mL
Storage & Handling :The antibody solution should be stored undiluted between 2°C and 8°C, and protected fromprolonged exposure to light. Do not freeze.
Application :FC - Quality tested
Recommended Usage :Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometricanalysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per millioncells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance foreach application.
Excitation Laser :Blue Laser (488 nm)、Green Laser (532 nm)、Yellow-Green Laser (561 nm)
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文献和实验1. Maier H, et al. 2007. J. Immunol. 178:2714.
2. Meng Q, et al. 2006. Invest. Ophthalmol. Vis. Sci. 47:4444. PubMed
3. Scarlett UK, et al. 2012. J Exp Med. 209:495. PubMed
4. Grabie N, et al. 2007. Circulation 116:2062. (IF, IHC)
5. Paterson AM, et al. 2011. J. Immunol. 187:1097.
6. Channappanavar R, et al. 2012. PLoS One 7:e39757. (Block)
7. Schreiber HA, et al. 2010. PLoS One 5:e11453. (Block) PubMed
8. Muthumani K, et al. 2011. J. Immunol. 187:2932. (Block) PubMed
9. Cripps JG, et al. 2010. Hepatology 52:1350. (Block) PubMed
10. Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
11. Riella LV, et al. 2011. Am. J. Transplant 11:832-40. (IHC)
12. Lei GS, et al. 2015. Infect Immun. 83:572. PubMed
抗体二抗上连接有荧光染料。直接标记染色背景低,实验程序少尽量减少实验工序和过程,以保证实验的真实和准确性。因此在条件允许的范围内,建议尽量用直接标记的抗体进行实验。 3、流式抗体荧光标记的选择: 如果实验中检测单一指标:不同荧光标记在不同的仪器上强度不同。以某仪器为例:PE >APC >PE-Cy5 >PerCP >FITC >PerCP-Cy5.5,通常来说,PE最强,适用于弱表达抗原。FITC强度较弱,适用于强表达抗原,使用范围比较广。用户需根据检测的目标蛋白进行具体选择。 如果同时检测多个
出现IgG 分子50 and 25 kDa 的重链和轻链条带。 5 二抗的选择 二抗应选用与使用的一抗相同的物种来源,例如:如果你的一抗是小鼠的单克隆抗体,二抗则选抗小鼠的二抗anti-mouse secondary。建议检查二抗说明书确保该抗体适用于你的检测应用, 二抗一般连接荧光素FITC 或发光团。 6 双重染色抗体的选择用未偶联一抗进行细胞培养物或组织切片的双重免疫染色要求一抗来源于不同物种并且二抗分别识别其中之一,二抗说明书应描述其与其它物种来源的免疫球蛋有否有交叉吸附。 想了解
被 PeterLinsley 鉴定,而且免疫抑制功能也是在 1994 年被 Jeffrey Bluestone 发现。但Allison 始终对外宣称他是发明了 CTLA-4 抗体治疗肿瘤的。James Allison 与他自己牌照为CTLA-4的保时捷 911 跑车而陈列平教授率先克隆并鉴定了 B7-H1 分子(即现在的 PD-L1)做出的开创工作(1999 年)。陈列平教授率先证明了 B7-H1 蛋白在许多人肿瘤里大量地表达,为临床试验奠定基础(2002 年)。Tasuku Honjo 这时才开始了解到 10 年前











