相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 供应商:
齐源
- 库存:
9999
- 靶点:
CD357
- 级别:
anti-human CD357 (GITR) Antibody
- 目录编号:
anti-human CD357 (GITR) Antibody
- 克隆性:
单克隆
- 抗原来源:
小鼠
- 保质期:
anti-human CD357 (GITR) Antibody
- 抗体英文名:
Purified anti-human CD357 (GITR) Antibody
- 抗体名:
CD357 (GITR) 抗体
- 标记物:
anti-human CD357 (GITR) Antibody
- 宿主:
小鼠
- 适应物种:
人
- 免疫原:
anti-human CD357 (GITR) Antibody
- 亚型:
anti-human CD357 (GITR) Antibody
- 形态:
anti-human CD357 (GITR) Antibody
- 应用范围:
FC
- 保存条件:
2-8℃
- 浓度:
0.5 mg/ml
- 规格:
100 ug
Description :GITR (glucocorticoid-induced TNF receptor family-regulated gene) is a 25 kD TNF receptorsuperfamily member (also known as AITR and TNFRSF18). GITR is expressed on activatedlymphocytes and is upregulated by T cell receptor engagement. The cytoplasmic domain ofGITR is homologous to CD40, 4-1BB and CD27 and has been shown to interact with TRAF 1-3, but not TRAF 5 or 6. GITR signaling has been shown to regulate T cell proliferation andTCR-mediated apoptosis, and to break immunological self-tolerance. GITR binds GITRL and isinvolved in the development of regulatory T cells and to regulate the activity of Th1 subsets.
Verified Reactivity :Human
Antibody Type :Monoclonal
Host Species :Mouse
Immunogen :Recombinant human GITR-Fc chimera
Formulation :Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation :The antibody was purified by affinity chromatography.
Concentration :0.5 mg/ml
Storage & Handling :The antibody solution should be stored undiluted between 2°C and 8°C.
Application :FC - Quality tested
Recommended Usage :Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometricanalysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cellsin 100 µl volume. It is recommended that the reagent be titrated for optimal performance for eachapplication.
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验1. Tavazoie MF, et al. 2018. Cell. 172:825. PubMed
2. Thrash EM, et al. 2020. STAR Protoc. 1:100055. PubMed
5.5, APC等。 常见荧光素波长分布示意图 同型对照的选择: 流式细胞实验和其他抗体相关实验有点不同的就是需要选择同型对照。同型对照,是用于消除由于抗体非特异性结合到细胞表面而产生的背景染色,相当于实验的阴性对照。同型对照选择与标记抗体同种属来源,同亚型,同荧光标记的抗体。比如:抗人的CD3的PE标记的抗体,小鼠的IgG2a。同型
非特异性结合到细胞表面而产生的背景染色,相当于实验的阴性对照。同型对照选择与标记抗体同种属来源,同亚型,同荧光标记的抗体。比如:抗人的CD3的PE标记的抗体,小鼠的IgG2a。同型对照选择PE标记的小鼠的IgG2a。 流式抗体使用注意事项: 1. 建议实验细胞的数量和抗体的比例要适当。细胞过量或抗体过量都可能使实验结果受影响,因此需要优化试验条件。注:不同的流式技术对抗体的需求量有较大差异,例如传统流式细胞术(采用鞘液流系统)需要1×106个细胞为起始上样量,抗体用量为10 μl
对照选择与标记抗体同种属来源,同亚型,同荧光标记的抗体。比如:抗人的CD3的PE标记的抗体,小鼠的IgG2a。同型对照选择PE标记的小鼠的IgG2a。 流式抗体使用注意事项: 1、建议实验细胞的数量和抗体的比例要适当。细胞过量或抗体过量都可能使实验结果受影响,因此需要优化试验条件。注:不同的流式技术对抗体的需求量有较大差异,例如传统流式细胞术 (采用鞘液流系统)需要1×106个细胞为起始上样量,抗体用量为10μl,而微流式细胞术 则只需5×105个细胞,抗体用量减少到5μl。 2、直标的流式抗体











