Description :CD184, also known as fusin or CXCR4, is a 45 kD seven transmembrane G-protein-linked CXCchemokine receptor. CD184 is widely expressed on blood and tissue cells, including B and Tcells, monocytes, macrophages, dendritic cells, granulocytes, megakaryocytes/platelets,lymphoid, myeloid precursor cells, endothelial cells, epithelial cells, astrocytes, and neurons,among other tissue cells. CD184 is the receptor for CXC chemokine SDF-1, mediates bloodcell migration, and is involved in B lymphopoiesis and myelopoiesis, cardiogenesis, bloodvessel formation, and cerebellar development. CXCR4 is also a coreceptor of X4 HIV-1 and analternative receptor for some isolates of HIV-2.
Formulation :Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 1 mM EDTA
Preparation :The antibody was purified by chromatography and conjugated with TotalSeq™-C oligomer underoptimal conditions.
Concentration :0.5 mg/ml
Storage & Handling :The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application :PG - Quality tested
Recommended Usage :Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometricanalysis and the oligomer sequence is confirmed by sequencing. TotalSeq™-C antibodies arecompatible with 10x Genomics Chromium Single Cell Immune Profiling Solution.
To maximize performance, it is strongly recommended that the reagent be titrated for eachapplication, and that you centrifuge the antibody dilution before adding to the cells at 14,000xg at 2- 8°C for 10 minutes. Carefully pipette out the liquid avoiding the bottom of the tube and add to thecell suspension. For Proteogenomics analysis, the suggested starting amount of this reagent fortitration is ≤ 1.0 µg per million cells in 100 µL volume. Refer to the corresponding TotalSeq™protocol for specific staining instructions.