BioLegend 801705 Alexa Fluor®

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BioLegend 801705 Alexa Fluor® 488 anti-Neurofilament H (NF-H), Nonphosphorylated
产品简介：
产品品牌：BioLegend
产品货号：801705
产品名称：BioLegend 801705 Alexa Fluor® 488 anti-Neurofilament H (NF-H), Nonphosphorylated
产品规格：25 µg

产品说明：
Clone   SMI 32 (See other available formats)
Regulatory Status   RUO
Other Names
Neurofilament heavy polypeptide, NF-H, 200 kD neurofilament protein, neurofilament triplet H protein
Isotype   Mouse IgG1

描述
神经丝（NF）是在神经元中发现的大约10纳米的中间丝。它们是神经元细胞骨架的主要组成部分，主要用于为轴突提供结构支持和调节轴突直径。有三个主要的NF亚基，这些亚基的名称是基于SDS-PAGE上哺乳动物亚基的表观分子量。轻或最低NF（NF-L）在68-70kD处运行。中等或中等NF（NF-M）在约145-160kD处运行，而重或最高NF（NF-H）在200-220kD处进行。然而，由于分子的高电荷C末端区域，这些蛋白质的实际分子量要低得多。NF基因表达水平与轴突直径相关，轴突直径控制着电信号沿轴突传播的速度。具有NF异常的突变小鼠具有类似肌萎缩侧索硬化症的表型。NF免疫染色在诊断神经病理学中很常见。它有助于区分神经元（NF阳性）和胶质细胞（NF阴性）。
Neurofilaments (NF) are approximately 10 nanometer intermediate filaments found in neurons. They are a major component of the neuronal cytoskeleton, and function primarily to provide structural support for the axon and to regulate the axon diameter. There are three major NF subunits, and the names given to these subunits are based upon the apparent molecular mass of the mammalian subunits on SDS-PAGE. The light or lowest NF (NF-L) runs at 68-70 kD. The medium or middle NF (NF-M) runs at about 145-160 kD, and the heavy or highest NF (NF-H) runs at 200-220 kD. However, the actual molecular weight of these proteins is considerably lower due to the highly charged C-terminal regions of the molecules. The level of NF gene expression correlates with the axonal diameter, which controls how fast electrical signals travel down the axon. Mutant mice with NF abnormalities have phenotypes resembling amyotrophic lateral sclerosis. NF immunostaining is common in diagnostic neuropathology. It is useful for differentiating neurons (positive for NF) from the glia (negative for NF).

Product Details
Verified Reactivity   Human, Mouse, Rat
Antibody Type   Monoclonal
Host Species  Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration   0.5 mg/ml

Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application   IHC-P - Quality tested

Recommended Usage
Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 5.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

Antigen Details
Structure
Neurofilament H has an apparent molecular mass of 200-220 kD.

Distribution
Tissue Distribution: CNS, peripheral nerves and glandular cells of the prostate
Cellular Distribution: cytoskeleton, nucleus, cytosol, and mitochondrion

Function
NF-H Neurofilaments are the major components of the neuronal cytoskeleton. They provide axonal support and regulate axon diameter. Phosphorylation of NF-H results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.

Ligand/Receptor
Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation result in changes to the neurofilament function.

Cell Type   Mature Neurons
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers
Molecular Family    Intermediate Filaments, Phospho-Proteins

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