BioLegend 126405 Alexa Fluor®

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BioLegend 126405 Alexa Fluor® 488 anti-mouse FOXP3
产品简介：
产品品牌：BioLegend
产品货号：126405
产品名称：BioLegend 126405 Alexa Fluor® 488 anti-mouse FOXP3
产品规格：25 µg

产品说明：
Clone  MF-14 (See other available formats)
Regulatory Status  RUO
Other Names  Forkhead box protein P3, Scurfin, JM2, IPEX, Zinc finger protein JM2
Isotype  Rat IgG2b, κ

描述
FOXP3是一种47 kD的转录因子，也称为叉头盒蛋白P3、Scurfin、JM2或IPEX。它被认为是T调节细胞的主调控基因和比大多数细胞表面标记物（如CD4和CD25）更特异的标记物。FOXP3在CD4+/CD25-细胞中的转导表达已被证明可诱导GITR、CD103和CTLA4并赋予T调节细胞表型。FOXP3在人类和“坏血病”小鼠的X连锁自身免疫过敏性失调综合征（XLAAD或IPEX）中发生突变。FOXP3的过表达已被证明导致低活性免疫状态，这表明该转录因子是T细胞活性的中心调节因子。
FOXP3 is a 47 kD transcription factor, also known as Forkhead box protein P3, Scurfin, JM2, or IPEX. It is proposed to be a master regulatory gene and more specific marker of T regulatory cells than most cell surface markers (such as CD4 and CD25). Transduced expression of FOXP3 in CD4+/CD25- cells has been shown to induce GITR, CD103, and CTLA4 and impart a T regulatory cell phenotype. FOXP3 is mutated in X-linked autoimmunity-allergic dysregulation syndrome (XLAAD or IPEX) in humans and in "scurfy" mice. Overexpression of FOXP3 has been shown to lead to a hypoactive immune state suggesting that this transcriptional factor is a central regulator of T cell activity.

Product Details
Isotype Control  Alexa Fluor® 488 Rat IgG2b, κ Isotype Ctrl
Verified Reactivity  Mouse
Antibody Type  Monoclonal
Host Species  Rat
Formulation  Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration  0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application  ICFC - Quality tested
IHC - Verified

Recommended Usage
Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear™ Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser  Blue Laser (488 nm)
Application Notes
NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended.

Antigen Details
Structure
50-55 kd protein. Forkhead/winged-helix transcription factor family, contains zinc finger and forkhead domains.
Distribution  Nuclear; expressed in Treg cells.
Function
Master regulatory gene in Treg cell development, crucial for immune homeostasis.
Interaction  Interacts with DNA
Cell Type  Tregs
Biology Area  Immunology
Molecular Family  Nuclear Markers

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