BioLegend 699308 Alexa Fluor®

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BioLegend 699308 Alexa Fluor® 647 anti-Vimentin
产品简介：
产品品牌：BioLegend
产品货号：699308
产品名称：BioLegend 699308 Alexa Fluor® 647 anti-Vimentin
产品规格：100 µg

产品说明：
Clone   W16220A (See other available formats)
Regulatory Status   RUO
Other Names
Vim, CTRCT30, HEL 113, Epididymis Luminal Protein 113
Isotype   Rat IgG2a, κ

描述
波形蛋白是在各种非上皮细胞，特别是间充质细胞中发现的III类中间丝。波形蛋白是一种广泛表达且高度保守的54kD蛋白，在间充质细胞、血管内皮细胞、肾小管细胞、巨噬细胞、中性粒细胞、成纤维细胞和白细胞中组成性表达1，2。波形蛋白被用作间充质细胞的标志物，以区分它们与上皮细胞3。波形蛋白表达增加经常被用作癌症中的EMT标志物4。波形蛋白自身抗体通常存在于狼疮5和类风湿性关节炎6等自身免疫性疾病患者中，也在移植后发现7。
Vimentin are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is a widely expressed and highly conserved 54 kD protein that is constitutively expressed in mesenchymal cells, endothelial cells lining blood vessels, renal tubular cells, macrophages, neutrophils, fibroblasts, and leukocytes1,2. Vimentin is used as a marker of mesenchymal cells to distinguish them from epithelial cells3. Increased vimentin expression is frequently used as an EMT marker in cancer4. Autoantibodies to vimentin are commonly found in patients with autoimmune diseases such as Lupus5 and rheumatoid arthritis6, and also found after transplantation7.

Product Details
Isotype Control   Alexa Fluor® 647 Rat IgG2a, κ Isotype Ctrl
Verified Reactivity   Mouse
Antibody Type   Monoclonal
Host Species   Rat

Immunogen
Partial mouse Vimentin (224-446 a.a.) recombinant protein expressed in E. coli.

Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide

Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration   0.5 mg/mL

Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application   ICC - Quality tested
ICFC - Verified

Recommended Usage
Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/mL is recommended. For intracellular flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

Antigen Details
Structure
466 amino acids with a predicted molecular weight of 54 kD.
Distribution   Cytoplasm.

Function
Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.

Interaction
HCV core protein, LGSN, SYNM, PLEC, SLC6A4, STK33, LARP6, RAB8B, TOR1A, TOR1AIP1, and BCAS3.

Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family    Intermediate Filaments

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