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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
齐源
- 库存:
999
- 靶点:
CD64
- 级别:
anti-human CD64
- 目录编号:
anti-human CD64
- 克隆性:
单克隆
- 抗原来源:
小鼠
- 保质期:
anti-human CD64
- 抗体英文名:
Biotin anti-human CD64 Antibody
- 抗体名:
CD64抗体
- 标记物:
anti-human CD64
- 宿主:
小鼠
- 适应物种:
人、猴
- 免疫原:
anti-human CD64
- 亚型:
anti-human CD64
- 形态:
anti-human CD64
- 应用范围:
FC
- 保存条件:
2-8℃
- 浓度:
0.5毫克/毫升
- 规格:
100 ug
CD64 is a 72 kD single chain type I glycoprotein also known as FcγRI and FcR I. CD64 is amember of the immunoglobulin superfamily and is expressed on monocytes/macrophages,dendritic cells, and activated granulocytes. The expression can be upregulated by IFN-γstimulation. CD64 binds IgG immune complex. It plays a role in antigen capture, phagocytosisof IgG/antigen complexes, and antibody-dependent cellular cytotoxicity (ADCC).
Verified Reactivity :Human, Cynomolgus, Rhesus
Reported Reactivity :Baboon, Capuchin Monkey, Chimpanzee, Squirrel Monkey
Antibody Type :Monoclonal
Host Species :Mouse
Immunogen :Human rheumatoid synovial fluid cells and fibronectin-purified monocytes.
Formulation :Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation :The antibody was purified by affinity chromatography, and conjugated with biotin under optimalconditions.
Concentration :0.5 mg/ml
Storage & Handling :The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application :FC - Quality tested
Recommended Usage :Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometricanalysis. For flow cytometric staining, the suggested use of this reagent is ≤2.0 µg per million cellsin 100 µl volume. It is recommended that the reagent be titrated for optimal performance for eachapplication.
Application Notes :Clone 10.1 recognizes the EC3 epitope of CD64. While both contain the EC3 domain, in-housetesting suggests that clone 10.1 preferentially binds to CD64A (FcγRIA), but not CD64B (FcγRIB).Additional reported applications (for the relevant formats) include: blocking of human IgG3 andmurine IgG2a binding to FcγRI and immunohistochemical staining of a.cetone-fixed frozentissue sections .2,5,6,1112
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文献和实验1. McMichael A, et al. Eds. 1987. Leucocyte Typing III. Oxford University Press. New York.
2. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. p. 874.
3. Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London.
4. Holl V, et al. 2004. J. Immunol. 173:6274.
5. Hober D, et al. 2002. J. Gen. Virol. 83:2169.
6. Cho HJ, et al. 2007. Physiol Genomics 149:60.
7. van Tits L, et al. 2005. Arterioscler Thromb Vasc Biol. 25:717. PubMed
8. Bruhns P, et al. 2008. Blood 113:3716. PubMed
9. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
10. Carter DL, et al. 1999. Cytometry 37:41. (FC)
11. Dougherty GJ, et al. 1987. Eur. J. Immunol. 17:1453.
12. Blom AB, et al. 2003. Arthritis Rheum. 48(4):1002-14. (IHC)
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