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- 齐源
- 342409
- 国产/进口
- 2025年07月16日
- PG
- 小鼠
- 人
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
齐源
- 库存：
999
- 靶点：
C5L2
- 级别：
C5L2 Antibody
- 目录编号：
C5L2 Antibody
- 克隆性：
单克隆
- 抗原来源：
小鼠
- 保质期：
C5L2 Antibody
- 抗体英文名：
TotalSeq™-C0572 anti-human C5L2 Antibody
- 抗体名：
C5L2 Antibody
- 标记物：
C5L2 Antibody
- 宿主：
小鼠
- 适应物种：
人
- 免疫原：
C5L2 Antibody
- 亚型：
C5L2 Antibody
- 形态：
C5L2 Antibody
- 应用范围：
PG
- 保存条件：
2-8℃
- 浓度：
0.5 mg/mL
- 规格：
10 ug
Description ：C5L2 (C5a receptor-like 2) is a seven-transmembrane orphan receptor, also known as GPR77,which shares significant homology to C5a receptor (CD88), but it is a decoy receptor withoutG-protein coupling. C5L2 is co-expressed with C5aR on many kinds of cells, such asneutrophils, immature dendritic cells, mast cells, macrophages, and astrocytes. C5L2 bindsC5a with similar high affinity as C5aR and relatively higher affinity for C5a-des-Arg than withC5aR.
Verified Reactivity ：Human
Reported Reactivity ：African Green, Baboon, Cynomolgus, Rhesus
Antibody Type ：Monoclonal
Host Species ：Mouse
Formulation ：Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 1 mM EDTA
Preparation ：The antibody was purified by chromatography and conjugated with TotalSeq™-C oligomer underoptimal conditions.
Concentration ：0.5 mg/mL
Storage & Handling ：The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application ：PG - Quality tested
Recommended Usage ：Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometricanalysis and the oligomer sequence is confirmed by sequencing. TotalSeq™-C antibodies arecompatible with 10x Genomics Chromium Single Cell Immune Profiling Solution.
To maximize performance, it is strongly recommended that the reagent be titrated for eachapplication, and that you centrifuge the antibody dilution before adding to the cells at 14,000xg at 2- 8°C for 10 minutes. Carefully pipette out the liquid avoiding the bottom of the tube and add to thecell suspension. For Proteogenomics analysis, the suggested starting amount of this reagent fortitration is ≤ 1.0 µg per million cells in 100 µL volume. Refer to the corresponding TotalSeq™protocol for specific staining instructions.
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文献和实验1. Fonseca MI, et al. 2013. J. Neuroinflammation. 10:25. (WB) PubMed
2. Hao J, et al. 2013. PLoS One 8:e66305. (Block)
3. Raby AC, et al. 2011. Eur. J. Immunol. 41:2741. (Block, WB)
（一）材料： 1．动物：家兔 2．人血清 3．羊毛脂、石蜡油、卡介苗 4．其它：无菌生理盐水，无菌研钵等 （二）方法： 1．弗氏 佐剂 的制备 将羊毛脂与石蜡油按1：5比例混合，高压灭菌。 2．人血清——弗氏完全的制备 将灭菌 佐剂 一份置 研钵 中，边研磨边滴加等量的含卡介苗3-4mg/mL的抗原
抗人肺腺癌单链抗体的构建 (1)将表达载体pFUW80质粒经XbaI和EcoRI双酶切，同样双酶切在pUC19中克隆的VL基因，分别回收后，连接并转化大肠杆菌JM109株，鉴定阳性重组子(可通过XbaI、EcoRI双酶切切下合适大小的带，即VL片段)。 (2)将上述经过鉴定的重组质粒用XhoI、SpeI双酶切，回收后，与同样双酶切的在pUC19中克隆的VH基因相连，再次转化JM109感受态细菌。重组质粒筛选、鉴定同上所述。至此抗人
沉淀和聚集，影响抗体的效价，随着先进技术的出现，冻干粉形式的抗体已经上市，如美天旎品牌的StainExpress™系列抗体可以在19−25°C环境下保存。 抗体选择 大多数只标明用于WB、IP、IHC、IF、ChIP的抗体都不适用于流式实验(FC,flowcytometry)。选择标明用于FC实验的抗体，在Panel设计上咨询专业人士，通过自己的实验结果进行改进，采用专业的工具进行配色辅助，文献和实验数据图作为参考。
