- ￥6800
- Tanda
- TD-83601
- USA
- 2025年12月25日
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
武汉天德
- 库存：
8000
- 英文名：
RalA Pull-Down Activation Assay Kit
- 保质期：
24个月
- 保存条件：
store at -20℃
- 规格：
30 Assays
RalA Pull-Down Activation Assay Kit
Cat. # TD-83601
Background
Small GTPases are a super-family of cellular signaling regulators. The Ras-like small G proteins, RalA/B, are important components of Ras signaling pathways, implicated in the initiation and maintenance of tumorigenic transformation, as well as vesicle transport, apoptosis, transcription, cell migration, and cell proliferation.
Currently there is no direct assay to measure the activation of Ral GTPases.
The RalA Activation Assay Kit is based on the configuration-specific monoclonal antibody that specifically recognizes Ral-GTP, but not Ral-GDP, and a RalA specific rabbit polyclonal antibody. Given the high affinity of monoclonal antibodies to their antigens, the activation assay could be performed in a short time. This assay provides the reliable results with consistent reproducibility.
These anti-Ral-GTP monoclonal antibodies can also be used to monitor the activation of Ral in cells and in tissues by immunohistochemistry.
Assay Principle
The RalA Activation Assay Kit uses configuration-specific anti-Ral-GTP Mouse monoclonal antibody to measure Ral-GTP levels either from cell extracts or from in vitro GTPγS loading Ral activation assays. Anti-Ral-GTP mouse monoclonal antibody is first incubated with cell lysates containing Ral-GTP. Next, the GTP-bound Ral is pulled down by protein A/G agarose. Finally, the precipitated active RalA or RalB will be detected by immunoblot analysis using anti- RalA or RalB rabbit polyclonal antibody, respectively.
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文献和实验Pull-Down Assay for Analysis of Integrin-Mediated Activation of Rap Proteins in Adherent Platelets
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因为自己实验后期需要使用这方面的技术,所以最近在查阅这方面的资料,想拿出来和大家一起分享.后期会把更详细的东西贴出来.英文水平一般,所以翻译的有点差,请海涵. Rac1 Activation Assay Kit被设计来鉴定贴壁细胞或非贴壁细胞的细胞裂解液中Rac活性数量(i.e. Rac-GTP, rather than the inactive Rac-GDP). 使用的glutathione (GST) pull-down方法，采用GST-agarose beads与有的包含74
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