- ￥6800
- Tanda
- TD-81701
- USA
- 2025年12月25日
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
武汉天德
- 库存：
8000
- 英文名：
Ran Pull-Down Activation Assay Kit
- 保质期：
24个月
- 保存条件：
store at -20℃
- 规格：
30 Assays
Ran Pull-Down Activation Assay Kit
Cat. # TD-81701
Background
Small GTPases are a super-family of cellular signaling regulators. Ran (Ras-related nuclear protein) is a member of the Ras-superfamily GTPases. Ran is involved in the transport of proteins across the nuclear envelope, as well as in microtubule organization during mitosis. Currently there is no direct assay to measure the activation of Ran GTPases.
The Ran Activation Assay Kit is based on the configuration-specific monoclonal antibody that specifically recognizes Ran-GTP, but not Ran-GDP. Given the high affinity of monoclonal antibodies to their antigens, the activation assay could be performed in a short time. This assay provides the reliable results with consistent reproducibility.
Assay Principle
The Ran Activation Assay Kit uses configuration-specific anti-Ran-GTP Mouse monoclonal antibody to measure Ran-GTP levels in cell extracts or in vitro GTPγS loading Ran activation assays. Anti-Ran-GTP mouse monoclonal antibody is first incubated with cell lysates containing Ran-GTP. Next, the GTP-bound Ran is pulled down by protein A/G agarose. Finally, the precipitated Ran-GTP is detected through immunoblot analysis using Anti-Ran Rabbit Polyclonal Antibody.
The anti-Ran-GTP monoclonal antibody can also be used to monitor the activation of Ran in cells and in tissues by immunohistochemistry.
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