- ￥6800
- Tanda
- TD-82801
- USA
- 2025年12月25日
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
武汉天德
- 库存：
8000
- 英文名：
Rab35 Pull-Down Activation Assay Kit
- 保质期：
24个月
- 保存条件：
store at -20℃
- 规格：
30 Assays
Rab35 Pull-Down Activation Assay Kit
Cat. # TD-82801
Background
The Rab family of GTPases regulates eukaryotic vesicular membrane traffic. Rab35 is with both plasma membrane and endosomal localization and has been implicated in diverse processes that include T-cell receptor recycling, oocyte yolk protein recycling and cytokinesis. Rab35 regulates neurite outgrowth in neuronal-like cells and can induce protrusions.
Currently there is no direct assay to measure the activation of Rab35 GTPases.
The Rab35 Activation Assay Kit is based on the configuration-specific monoclonal antibody that specifically recognizes Rab35-GTP, but not Rab35-GDP. Given the high affinity of monoclonal antibodies to their antigens, the activation assay could be performed in a short time. This assay provides the reliable results with consistent reproducibility.
Assay Principle
The Rab35 Activation Assay Kit uses configuration-specific anti-Rab35-GTP Mouse monoclonal antibody to measure Rab35-GTP levels in cell extracts or in vitro GTPγS loading Rab35 activation assays. Anti-Rab35-GTP mouse monoclonal antibody is first incubated with cell lysates containing Rab35-GTP. Next, the GTP-bound Rab35 is pulled down by protein A/G agarose. Finally, the precipitated Rab35-GTP is detected through immunoblot analysis using Anti-Rab35 Rabbit Polyclonal Antibody.
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文献和实验Pull-Down Assay for Analysis of Integrin-Mediated Activation of Rap Proteins in Adherent Platelets
high levels of Rap1 proteins, mainly Rap1b, which plays a critical role in platelet adhesion and activation. Rap1 is a key element in the inside-out signaling pathway leading to the conversion of integrins into the high-affinity
因为自己实验后期需要使用这方面的技术,所以最近在查阅这方面的资料,想拿出来和大家一起分享.后期会把更详细的东西贴出来.英文水平一般,所以翻译的有点差,请海涵. Rac1 Activation Assay Kit被设计来鉴定贴壁细胞或非贴壁细胞的细胞裂解液中Rac活性数量(i.e. Rac-GTP, rather than the inactive Rac-GDP). 使用的glutathione (GST) pull-down方法，采用GST-agarose beads与有的包含74
Quantifying Sphingosine-1-Phosphate-Dependent Activation of the RhoGTPases
to monitor S1P-dependent activation of the Rho family GTPases is critical for our understanding of S1P signaling. Herein, we provide methods for the GST pull-down-based assay used to measure Rho, Rac, and Cdc42 activity in cultured cells treated with S1P.
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