- ￥6800
- Tanda
- TD-82401
- USA
- 2026年04月21日
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
武汉天德
- 库存：
8000
- 英文名：
Arf6 Pull-Down Activation Assay Kit
- 保质期：
24个月
- 保存条件：
store at -20℃
- 规格：
30 Assays
Arf6 Pull-Down Activation Assay Kit
Cat. # TD-82401
Background
ARF6 (ADP-ribosylation factor 6) is a member of the ARF super-family. ARF genes encode small GTPases that increase the ADP-ribosyltransferase activity of cholera toxin and are critical for vesicular trafficking as activators of phospholipase D. Arf6 regulates membrane trafficking and functions as a regulatory molecule of phagocytosis.
Currently there is no direct assay to measure the activation of Arf6 GTPase.
Assay Principle
The Arf6 Activation Assay Kit uses configuration-specific anti-Arf6-GTP Mouse monoclonal antibody to measure Arf6-GTP levels in cell extracts or in vitro GTPγS loading Arf6 activation assays. Anti-Arf6-GTP mouse monoclonal antibody is first incubated with cell lysates containing Arf6-GTP. Next, the GTP-bound Arf6 is pulled down by protein A/G agarose. Finally, the precipitated Arf6-GTP is detected through immunoblot analysis using Anti-Arf6 Rabbit Polyclonal Antibody.
The anti-Arf6-GTP monoclonal antibody can also be used to monitor the activation of Arf6 in cells and in tissues by immunohistochemistry.
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文献和实验Pull-Down Assay for Analysis of Integrin-Mediated Activation of Rap Proteins in Adherent Platelets
high levels of Rap1 proteins, mainly Rap1b, which plays a critical role in platelet adhesion and activation. Rap1 is a key element in the inside-out signaling pathway leading to the conversion of integrins into the high-affinity
因为自己实验后期需要使用这方面的技术,所以最近在查阅这方面的资料,想拿出来和大家一起分享.后期会把更详细的东西贴出来.英文水平一般,所以翻译的有点差,请海涵. Rac1 Activation Assay Kit被设计来鉴定贴壁细胞或非贴壁细胞的细胞裂解液中Rac活性数量(i.e. Rac-GTP, rather than the inactive Rac-GDP). 使用的glutathione (GST) pull-down方法，采用GST-agarose beads与有的包含74
Quantifying Sphingosine-1-Phosphate-Dependent Activation of the RhoGTPases
to monitor S1P-dependent activation of the Rho family GTPases is critical for our understanding of S1P signaling. Herein, we provide methods for the GST pull-down-based assay used to measure Rho, Rac, and Cdc42 activity in cultured cells treated with S1P.
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