- ￥7800
- Tanda
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- TD-81701
- 2025年11月10日
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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
Ran Pull-Down Activation Assay Kit
- 保质期：
24个月
- 保存条件：
store at -20℃
- 库存：
8000
- 供应商：
武汉天德
- 规格：
30 Assays
Ran Pull-Down Activation Assay Kit
Cat. # TD-81701
Background
Small GTPases are a super-family of cellular signaling regulators. Ran (Ras-related nuclear protein) is a member of the Ras-superfamily GTPases. Ran is involved in the transport of proteins across the nuclear envelope, as well as in microtubule organization during mitosis. Currently there is no direct assay to measure the activation of Ran GTPases.
The Ran Activation Assay Kit is based on the configuration-specific monoclonal antibody that specifically recognizes Ran-GTP, but not Ran-GDP. Given the high affinity of monoclonal antibodies to their antigens, the activation assay could be performed in a short time. This assay provides the reliable results with consistent reproducibility.
Assay Principle
The Ran Activation Assay Kit uses configuration-specific anti-Ran-GTP Mouse monoclonal antibody to measure Ran-GTP levels in cell extracts or in vitro GTPγS loading Ran activation assays. Anti-Ran-GTP mouse monoclonal antibody is first incubated with cell lysates containing Ran-GTP. Next, the GTP-bound Ran is pulled down by protein A/G agarose. Finally, the precipitated Ran-GTP is detected through immunoblot analysis using Anti-Ran Rabbit Polyclonal Antibody.
The anti-Ran-GTP monoclonal antibody can also be used to monitor the activation of Ran in cells and in tissues by immunohistochemistry.
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文献和实验因为自己实验后期需要使用这方面的技术,所以最近在查阅这方面的资料,想拿出来和大家一起分享.后期会把更详细的东西贴出来.英文水平一般,所以翻译的有点差,请海涵. Rac1 Activation Assay Kit被设计来鉴定贴壁细胞或非贴壁细胞的细胞裂解液中Rac活性数量(i.e. Rac-GTP, rather than the inactive Rac-GDP). 使用的glutathione (GST) pull-down方法，采用GST-agarose beads与有的包含74
1, For cells cultured in 2D, about 1-2X107 S1 cells were growth in 100mm dish, and were cross-linked by adding formaldehyde to final concentration of 1% and incubated in room temperature for 10 minutes. For cells growth in 3D, cells
Affinity-Based Assay of Rho Guanosine Triphosphatase Activation
The recognition that Rho guanosine triphosphatases (GTPases) (Rho, Rac, and Cdc42) play important regulatory roles in many areas of cell biology has made the ability to measure their activity in cells an important biological tool. Because Rho
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