- ￥6800
- Tanda
- TD-83401
- USA
- 2025年12月25日
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
武汉天德
- 库存：
8000
- 英文名：
Rab5B Pull-Down Activation Assay Kit
- 保质期：
24个月
- 保存条件：
store at -20℃
- 规格：
30 Assays
Rab5B Pull-Down Activation Assay Kit
Cat. # TD-83401
Background
Small GTPases are a super-family of cellular signaling regulators. Rab5 is a member of the Rab-family GTPases. Rab5 is a rate-limiting component of the machinery regulating the kinetics of membrane traffic in the early endocytic pathway.
Currently there is no direct assay to measure the activation of Rab5B GTPases.
Rab5B Activation Assay Kit is based on the configuration-specific monoclonal antibody that specifically recognizes Rab5-GTP, but not Rab5-GDP, and a Rab5B specific rabbit polyclonal antibody. Given the high affinity of monoclonal antibodies to their antigens, the activation assay could be performed in a short time. This assay provides the reliable results with consistent reproducibility.
Assay Principle
The Rab5B Activation Assay Kit uses configuration-specific anti-Rab5-GTP Mouse monoclonal antibody to measure Rab5-GTP levels in cell extracts or in vitro GTPγS loading Rab5 activation assays. Anti-Rab5-GTP mouse monoclonal antibody is first incubated with cell lysates containing Rab5-GTP. Next, the GTP-bound Rab5 is pulled down by protein A/G agarose. Finally, the precipitated Rab5-GTP is detected through immunoblot analysis using Anti-Rab5B Rabbit Polyclonal Antibody.
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文献和实验Pull-Down Assay for Analysis of Integrin-Mediated Activation of Rap Proteins in Adherent Platelets
high levels of Rap1 proteins, mainly Rap1b, which plays a critical role in platelet adhesion and activation. Rap1 is a key element in the inside-out signaling pathway leading to the conversion of integrins into the high-affinity
因为自己实验后期需要使用这方面的技术,所以最近在查阅这方面的资料,想拿出来和大家一起分享.后期会把更详细的东西贴出来.英文水平一般,所以翻译的有点差,请海涵. Rac1 Activation Assay Kit被设计来鉴定贴壁细胞或非贴壁细胞的细胞裂解液中Rac活性数量(i.e. Rac-GTP, rather than the inactive Rac-GDP). 使用的glutathione (GST) pull-down方法，采用GST-agarose beads与有的包含74
Quantifying Sphingosine-1-Phosphate-Dependent Activation of the RhoGTPases
to monitor S1P-dependent activation of the Rho family GTPases is critical for our understanding of S1P signaling. Herein, we provide methods for the GST pull-down-based assay used to measure Rho, Rac, and Cdc42 activity in cultured cells treated with S1P.
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