- ￥7800
- Tanda
- USA
- TD-83401
- 2025年11月10日
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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
Rab5B Pull-Down Activation Assay Kit
- 保质期：
24个月
- 保存条件：
store at -20℃
- 库存：
8000
- 供应商：
武汉天德
- 规格：
30 Assays
Rab5B Pull-Down Activation Assay Kit
Cat. # TD-83401
Background
Small GTPases are a super-family of cellular signaling regulators. Rab5 is a member of the Rab-family GTPases. Rab5 is a rate-limiting component of the machinery regulating the kinetics of membrane traffic in the early endocytic pathway.
Currently there is no direct assay to measure the activation of Rab5B GTPases.
Rab5B Activation Assay Kit is based on the configuration-specific monoclonal antibody that specifically recognizes Rab5-GTP, but not Rab5-GDP, and a Rab5B specific rabbit polyclonal antibody. Given the high affinity of monoclonal antibodies to their antigens, the activation assay could be performed in a short time. This assay provides the reliable results with consistent reproducibility.
Assay Principle
The Rab5B Activation Assay Kit uses configuration-specific anti-Rab5-GTP Mouse monoclonal antibody to measure Rab5-GTP levels in cell extracts or in vitro GTPγS loading Rab5 activation assays. Anti-Rab5-GTP mouse monoclonal antibody is first incubated with cell lysates containing Rab5-GTP. Next, the GTP-bound Rab5 is pulled down by protein A/G agarose. Finally, the precipitated Rab5-GTP is detected through immunoblot analysis using Anti-Rab5B Rabbit Polyclonal Antibody.
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文献和实验因为自己实验后期需要使用这方面的技术,所以最近在查阅这方面的资料,想拿出来和大家一起分享.后期会把更详细的东西贴出来.英文水平一般,所以翻译的有点差,请海涵. Rac1 Activation Assay Kit被设计来鉴定贴壁细胞或非贴壁细胞的细胞裂解液中Rac活性数量(i.e. Rac-GTP, rather than the inactive Rac-GDP). 使用的glutathione (GST) pull-down方法，采用GST-agarose beads与有的包含74
1, For cells cultured in 2D, about 1-2X107 S1 cells were growth in 100mm dish, and were cross-linked by adding formaldehyde to final concentration of 1% and incubated in room temperature for 10 minutes. For cells growth in 3D, cells
Affinity-Based Assay of Rho Guanosine Triphosphatase Activation
The recognition that Rho guanosine triphosphatases (GTPases) (Rho, Rac, and Cdc42) play important regulatory roles in many areas of cell biology has made the ability to measure their activity in cells an important biological tool. Because Rho
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