BioLegend 652408 Alexa Fluor®

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BioLegend 652408 Alexa Fluor® 647 anti-mouse Ki-67
产品简介：
产品品牌：BioLegend
产品货号：652408
产品名称：BioLegend 652408 Alexa Fluor® 647 anti-mouse Ki-67
产品规格：100 µg

产品说明：
Clone   16A8 (See other available formats)
Regulatory Status   RUO
Other Names   KIA, proliferation-related Ki-67 antigen
Isotype   Rat IgG2a, κ

描述
核蛋白Ki-67首先由单克隆抗体Ki-67鉴定，该抗体通过用L428霍奇金淋巴瘤细胞系的细胞核免疫小鼠而产生。Ki-67蛋白在核糖体RNA转录和细胞增殖中起着重要作用。Ki-67的表达发生在G1、S、G2和M期，而在G0期Ki-67蛋白是不可检测的。Ki-67在增殖细胞中强烈表达，已被报道为各种肿瘤的预后标志物。
The nuclear protein Ki-67 was first identified by the monoclonal antibody Ki-67, which was generated by immunizing mice with nuclei of the L428 Hodgkin lymphoma cell line. Ki-67 protein plays an essential role in ribosomal RNA transcription and cell proliferation. Expression of Ki-67 occurs during G1, S, G2, and M phase, while in G0 phase the Ki-67 protein is not detectable. Ki-67 is strongly expressed in proliferating cells and has been reported as a prognostic marker in various tumors.

Product Details
Isotype Control  Alexa Fluor® 647 Rat IgG2a, κ Isotype Ctrl
Verified Reactivity   Mouse
Antibody Type   Monoclonal
Host Species   Rat
Immunogen
E. coli expressed partial mouse Ki-67 recombinant protein, 1816-2163 aa.

Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.

Concentration   0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application   ICFC - Quality tested

Recommended Usage
Each lot of this antibody is quality control tested by our Ki-67 staining protocol below. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

Antigen Details
Structure
325 kD protein containing a forkhead-associated domain (FHA) and 13 tandem repeats
Distribution   Nucleus and chromosome

Function
Required for cell cycle progression and proliferation

Interaction
Interacts with KIF15; binds to MKI67IP through FHA domain
Biology Area
Cell Biology, Cell Cycle/DNA Replication, Transcription Factors
Molecular Family   Nuclear Markers

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