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- 文献和实验
- 技术资料
- 库存:
99
- 英文名:
Brilliant Violet 510™ Donkey anti-rabbit IgG (min. x-reactivity)
- 供应商:
北京云肽生物科技有限公司
北京云肽生物科技有限公司作为 BioLegend 的特约经销商,为广大客户提供原装正品,货期短,折扣好的服务准则。
美国 BioLegend 公司位于美国加州,致力于为生物医药研究的各个前沿领域提供高质量的抗体及其它相关试剂。BioLegend 虽然成立的时间不长,但它聚集了一批来自 PharMingen 公司的专业人士,建立了一支具有雄厚技术背景的专业队伍。通过其自身的先天优势,以及与世界知名实验室和研究所的良好合作关系,以合理的价格为广大客户提供高质量的免疫学和细胞生物学产品。
BioLegend 公司以其专业技术力量为保证,提供各种质量卓越的产品,并不断地开发新产品,以满足飞速发展的生命科学研究的需要。
BioLegend 406419 Brilliant Violet 510™ Donkey anti-rabbit IgG (min. x-reactivity)
产品简介:
产品品牌:BioLegend
产品货号:406419
产品名称:BioLegend 406419 Brilliant Violet 510™ Donkey anti-rabbit IgG (min. x-reactivity)
产品规格:50 µg
产品说明:
Clone Poly4064 (See other available formats)
Regulatory Status RUO
Isotype Donkey Polyclonal Ig
描述
这种多克隆驴抗兔 IgG 抗体与兔 IgG 的重链和大多数兔免疫球蛋白常见的轻链发生反应。未检测到针对非免疫球蛋白血清蛋白的交叉反应。该抗体已被固相吸收,以确保与大鼠、人、牛、马和小鼠免疫球蛋白的交叉反应最小,但它可能与兔免疫球蛋白的其他亚类发生交叉反应。
This polyclonal donkey anti-rabbit IgG antibody reacts with the heavy chains of rabbit IgG and with the light chains common to most rabbit immunoglobulins. No cross-reactivity has been detected against non-immunoglobulin serum proteins. This antibody has been solid-phase absorbed to ensure minimal cross-reaction with rat, human, bovine, horse, and mouse immunoglobulins, but it may cross-react with other subclasses of rabbit immunoglobulins.
Product Details
Verified Reactivity Rabbit
Antibody Type Polyclonal
Host Species Donkey
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 510™ under optimal conditions.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application FC - Quality tested
Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 510™ excites at 405 nm and emits at 510 nm. The bandpass filter 510/50 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 510™ is a trademark of Sirigen Group Ltd.
以下是 BioLegend 品牌部分产品,如需其他产品请联系本公司销售。
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BioLegend 326506 PE anti-human CD163 RM3/1 100 tests
BioLegend 326507 Alexa Fluor® 647 anti-human CD163 RM3/1 25 tests
BioLegend 326508 Alexa Fluor® 647 anti-human CD163 RM3/1 100 tests
BioLegend 326509 APC anti-human CD163 RM3/1 25 tests
BioLegend 326510 APC anti-human CD163 RM3/1 100 tests
BioLegend 326511 PerCP/Cyanine5.5 anti-human CD163 RM3/1 25 tests
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BioLegend 326513 PE/Cyanine7 anti-human CD163 RM3/1 25 tests
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文献和实验XXXX原代细胞干预培养与激光共聚焦蛋白细胞定位实验 实验试剂:PBS(磷酸盐缓冲液pH7.4)(实验室常规配置);非免疫山羊血清;Triton X-100(sigma);BSA抗体稀释液(实验室常规配置);A蛋白抗体(Santa cruz);B蛋白抗体(Abcam);Alexa Fluor® 488 donkey anti-goat IgG (H+L)(Invitrogen);Alexa Fluor® 555 Anti-Rabbit IgG (H+L), F(ab
, 5% BSA, pH 7.4) for 2 h at room temperature and then probed for CTGF by incubation for 40 min with anti-CTGF antibody at 0.5 µg/ml in blocking buffer. After extensive washing at 37°C, the filters were incubated with either HRP-conjugated donkey anti
Brdu免疫组织化学染色分析 BrdU incorporation assay
of Rhodamine(Tritc) conjugated Donkey anti mouse IgG with 1% NDS,1%BSA in PBS, incubate at RT for 1 hr. Wash in PBS 3x Mount with 50% glycerol in PBS. Culture Cell Enzyme Immunostaining for BrdU: Wash plates with in PBS
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