BioLegend 776606 Recombinant M

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BioLegend 776606 Recombinant Mouse CD112R-Fc Chimera (carrier-free)
产品简介：
产品品牌：BioLegend
产品货号：776606
产品名称：BioLegend 776606 Recombinant Mouse CD112R-Fc Chimera (carrier-free)
产品规格：100 µg

产品说明：
Regulatory Status   RUO
Other Names
Poliovirus receptor-related immunoglobulin domain containing protein, PVRIG, C7orf15, CD112 receptor

描述
CD112R基因编码单个跨膜蛋白，该蛋白由单个细胞外IgV结构域、一个跨膜结构域和一个包含两个酪氨酸残基的长细胞内结构域组成，其中一个位于ITIM样基序内。CD112R最初被命名为PVRIG，因为在其第二外显子与脊髓hui质炎病毒受体（PVR/CD155）和脊髓hui质炎病毒受体样（PVRL）基因的可变免疫球蛋白结构域之间观察到同源性。CD112R是CD112/Nectin-2的细胞表面受体，并且可以作为抑制T细胞受体介导的信号的共抑制受体。在与CD112相互作用后，CD112R抑制T细胞增殖，而CD112R和CD112之间相互作用的破坏增强了T细胞反应，这表明CD112R是人类T细胞的新检查点。CD112R与CD226竞争CD112结合。已经表明，CD112R的阻断增强了曲妥珠单抗（治疗具有高Her2表达的癌症的一线药物）触发的人类NK细胞的抗肿瘤反应。
CD112R gene encodes a single transmembrane protein consisting of a single extracellular IgV domain, one transmembrane domain, and a long intracellular domain which contains two tyrosine residues, one within an ITIM-like motif. CD112R was initially named PVRIG for the homology observed between its second exon and the variable immunoglobulin domain of the 脊髓hui质炎病毒 receptor (PVR/CD155) and 脊髓hui质炎病毒 receptor-like (PVRL) genes. CD112R is a cell surface receptor for CD112/Nectin-2 and may act as a coinhibitory receptor that suppresses T-cell receptor-mediated signals. Following interaction with CD112, CD112R inhibits T-cell proliferation and the disruption of interaction between CD112R and CD112 enhances T cell response, suggesting CD112R is a novel checkpoint for human T cells. CD112R competes with CD226 for CD112 binding. It has been shown the blockade of CD112R enhances Trastuzumab (first-line drug to treat breast cancer with high Her2 expression) triggered antitumor response by human NK cells.

Product Details
Source
Mouse CD112R, amino acids Ser35-Asp165 (Accession # A0A1B0GS01) with a C-terminal mouse IgG2a-Fc and 8-His tag was expressed in CHO cells.

Molecular Mass
The 384 amino acid recombinant protein has a predicted molecular mass of approximately 42.7 kD. The DTT-reduced protein migrates at approximately 50 kDa and non-reduced protein migrates at approximately 90 kD by SDS-PAGE. The predicted N-terminal amino acid is Ser.

Purity
> 95%, as determined by Coomassie stained SDS-PAGE.
Formulation
0.22 µm filtered protein solution is in PBS, pH 7.2

Endotoxin Level
Less than 0.1 EU per µg protein as determined by the LAL method.

Concentration
10-25 µg sizes are bottled at 200 µg/mL. 100 µg size is bottled at the concentration indicated on the vial.

Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.

Activity
When mouse CD112R is immobilized at 1.0 µg/mL, biotinylated mouse CD112 binds with EC50 of 15 - 60 ng/mL.
Application   Bioassay

Antigen Details
Structure   Homodimer
Distribution
Preferentially expressed in lymphocytes, such as T cells and NK cells, but not in monocyte derived dendritic cells.

Function
Cell surface receptor for CD112 that may act as a coinhibitory receptor that suppresses T-cell receptor-mediated signals.
Interaction   T cell, NK cells
Ligand/Receptor   CD112/Nectin-2
Bioactivity   Measured by its ability to bind mouse CD112
Cell Type   NK cells, T cells
Biology Area
Angiogenesis, Cancer Biomarkers, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology

Molecular Family
Adhesion Molecules, Immune Checkpoint Receptors, Soluble Receptors, TCRs

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