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- BioLegend
- 2025年10月31日
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- 库存：
99
- 英文名：
PE anti-human LGR5 (GPR49)
- 供应商：
北京云肽生物科技有限公司
北京云肽生物科技有限公司作为 BioLegend 的特约经销商，为广大客户提供原装正品，货期短，折扣好的服务准则。
美国 BioLegend 公司位于美国加州，致力于为生物医药研究的各个前沿领域提供高质量的抗体及其它相关试剂。BioLegend 虽然成立的时间不长，但它聚集了一批来自 PharMingen 公司的专业人士，建立了一支具有雄厚技术背景的专业队伍。通过其自身的先天优势，以及与世界知名实验室和研究所的良好合作关系，以合理的价格为广大客户提供高质量的免疫学和细胞生物学产品。
BioLegend 公司以其专业技术力量为保证，提供各种质量卓越的产品，并不断地开发新产品，以满足飞速发展的生命科学研究的需要。
BioLegend 373803 PE anti-human LGR5 (GPR49)
产品简介：
产品品牌：BioLegend
产品货号：373803
产品名称：BioLegend 373803 PE anti-human LGR5 (GPR49)
产品规格：25 tests
产品说明：
Clone SA222C5 (See other available formats)
Regulatory Status RUO
Other Names
G-Protein coupled receptor 49 (GPR49), G-Protein coupled receptor 67 (GPR67), FEX, HG38
Isotype Mouse IgG2a, κ
描述
LGR5，也称为GPR49，是一种100 KD的蛋白质。它是GPCR A类孤儿受体蛋白的成员，其结合R-spondin并且不像其他GPCR那样通过异源三聚体G蛋白激活。它触发下游的Wnt信号通路。LGR5在肌肉、胎盘、脊髓和大脑等多种组织中表达，特别是作为某些组织中成体干细胞的生物标志物。
LGR5, also known as GPR49, is a 100 KD protein. It is a member of GPCR class A orphan receptor proteins which binds R-spondin and does not activate through heterotrimeric G protein like other GPCR. It triggers the Wnt signaling pathway downstream. LGR5 is expressed across a diverse range of tissue such as in the muscle, placenta, spinal cord and brain and particularly as a biomarker of adult stem cells in certain tissues.
Product Details
Isotype Control PE Mouse IgG2a, κ Isotype Ctrl (FC)
Verified Reactivity Human
Antibody Type Monoclonal
Host Species Mouse
Immunogen Human LGR5 transfectant cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application FC - Quality tested
Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Antigen Details
Structure G-protein coupled receptor (GPCR) superfamily
Distribution
Muscle, placenta, spinal cord, brain and particularly as a biomarker of adult stem cells in certain tissues.
Function Triggers Wnt signaling pathway.
Interaction Wnt pathway
Ligand/Receptor R-spondins (RSpo 1,2,3, and 4)
Biology Area Immunology
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文献和实验Live‐Cell Immunofluorescence Staining of Human Pluripotent Stem Cells
) mCD49e BD PE (557447) 1:100
度的功能细胞。二、人外周血中 CD4 T 细胞分选实例1.产品信息： MojoSort™ Human CD4 T Cell Isolation Kit（BioLegend, Cat#480009），阴性分选试剂盒2.检测仪器型号：BD LSRⅡ3.样本：正常人外周血4.分选前后检测相关产品：PE-CD4（Biolegend, Cat# 317410）5.实验过程（1）采血之后采用达优™ 人淋巴细胞分离液分离人外周血单个核细胞。分离之后能见到明显的白膜层。（达优，Cat#DKW-KLSH-0100
Cell Surface Immunofluorescence Staining Protocol
实验试剂 Cell Staining Buffer [ Details:BioLegend Cat. #420201] Red Cell Lysis Buffer [ Details: BioLegend Cat. #420301] 7-AAD Viability Staining Solution[ Details: BioLegend Cat. #420403] TruStain FcX™[ Details: anti
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