Mouse IgG2b kappa, Isotype Con

Rapid Isotyping of Mouse Monoclonal Antibodies

for 5% of mouse antibodies) has limited practical value. In contrast, the heavy-chain isotype is very informative (see Table 1 ); protein A or protein G binding, euglobulin properties, complement binding, kinetics of digestion with papain or pepsin, clearance

Purification of Lin-, c-kit+, Sca-1+ bone marrow cells for Culture, Flow Cytometry, or Transplantaion

D  Granulocytic : Gr-1 (Ly-6G) IgG2b, 01211A  D8 IgG2b, 09821D  Eryth: Ter-119 IgG2b, 09081A  Sca-1 PE-IgG2a (E31-161.7) 01835B  PE-IgG2a Isotype control, 11025

Notes on Making Rat x Y3 Monoclonal Antibody Producing Hybridomas

me why this works!) before cloning out for variants. b) You can screen by ELISA or with mAb coupled RBC using mouse monoclonal antibodies against the Y3 derived Kappa 1a (RG11/15.5) and the specific DA Kappa 1b (G9/1.3), or the appropriate heavy chain (eg NORIG