RNA Seq建库试剂盒

BioDynami RNA Seq建库试剂盒

产品简介：

BioDynami开发了RNA Seq建库试剂盒，用于构建高质量的二代测序文库(illumina和MGI平台)。RNA测序是分析转录组的一个非常强大的工具，如基因表达和转录调控、剪接鉴定、突变和变异检测等。该试剂盒需要纯化的RNA(例如:去除rRNA的RNA或polyA mRNA)作为文库构建的输入。库多路复用可以使用不同类型的索引。RNAseq 主要研究的是 mRNA，由于一次转录过程中，mRNA 只占很少一部分（约 4~5%），需要采用特殊的建库方式将 mRNA 从总 RNA 中分离出来。
The RNA Seq Library Prep Kit was developed for construction of high quality NGS  libraries for next generation sequencing (illumina and MGI Platforms). RNA Sequencing is a very powerful tool to analyze transcriptome such as gene expression and transcription regulation, splicing characterization, mutation and variation detection etc. The kit needs purified RNA (example: rRNA depleted RNA or polyA mRNA) as input for library construction. Library multiplexing is possible with different types of indexes.

RNA Seq建库试剂盒工作流程

illumina平台试剂盒有三种index类型:

Non-index(illumina Cat.# 30055)：没有索引。

Index(illumina Cat.# 30056)：每个引物都包含6个碱基的独特序列。RNA测序文库复用可用于多达48个样本。

Unique dual index(illumina Cat.# 30057)：使用独特的双索引，可实现多达96个样本的RNA测序文库复用。建立了4-Base Difference Index System。该系统可以在8个碱基索引区域生成至少4个不同于其他碱基的索引。独特的双索引引物可识别测序错误，如索引跳变、分配错误和解复用错误。

MGI平台试剂盒(Cat.# 34112)亦适用上述index类型。

Three index types are available for the illumina platform kits:

Non-index (illumina Cat.# 30055): Libraries do not have index.

Index (illumina Cat.# 30056): A unique barcode sequence with 6 bases has been included in each of the index primers. RNA Sequencing library multiplexing is possible with up to 48 samples.

Unique dual index (illumina Cat.# 30057): RNA Sequencing library multiplexing up to 96 samples is possible with the unique dual indexes. We have developed a 4-Base Difference Index System. The system can generate indexes with at least 4 bases different from others in the 8-base indexing region. the unique dual indexing primers identify sequencing errors such as index hopping, mis-assignment, and de-multiplexing errors.

Indexes are available for the MGI platform kits (Cat.# 34112).

试剂盒特性：

★  快速的实验方案

★  保证质量

★  工作流程简单

★  输入纯化RNA量:从3 ng到100 ng

★  文库将在两小时内准备就绪

★  操作时间只有大约10分钟

★  高收益

★  模式统一

Features

• • • Quick protocol
      • Libraries will be ready in 2 hours
      • Hands-on time is only ~10 minutes
    • Guaranteed quality
      • High yield
      • Uniform coverage of transcripts
    • Simple workflow
    • Input purified RNA amount: From 3 ng to 100 ng

实验时间的比较:BioDynami RNA Seq Library Prep Kit与其他厂商的试剂盒。记录了操作时间和离开时间。

BioDynami试剂盒文库大小及分布，输入polyA mRNA 20 ng和3 ng。

相同条件下文库产量和重复率的比较:以20 ng和3 ng的polyA mRNA作为输入。测定PCR周期数。

比较转录本的覆盖率:20 ng的polyA mRNA作为输入。BioDynami试剂盒覆盖范围更均匀。

RNAseq 主要研究的是 mRNA，由于一次转录过程中，mRNA 只占很少一部分（约 4~5%），需要采用特殊的建库方式将 mRNA 从总 RNA 中分离出来。常用的方式有两种，一种是根据 PlolyA 尾巴进行富集，另一种是降解 rRNA 的方法。

产品订购：

产品名称	货号	产品规格
RNA Seq Library Prep Kit	30055S	non-index，24 reactions
RNA Seq Library Prep Kit	30055L	non-index，48 reactions
RNA Seq Library Prep Kit	30056S	index，24 reactions
RNA Seq Library Prep Kit	30056L	index，48 reactions
RNA Seq Library Prep Kit	30057S	unique dual index，96 reactions
RNA Seq Library Prep Kit	30057L	unique dual index，192 reactions
RNA Seq Library Prep Kit	34056S	index，24 reactions
RNA Seq Library Prep Kit	34056L	index，96 reactions