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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃/-80℃
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Cat Adiponectin (apM1)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
1mg/100ug/20ug
| 规格: | 1mg | 产品价格: | ¥15792.0 |
|---|---|---|---|
| 规格: | 100ug | 产品价格: | ¥5370.0 |
| 规格: | 20ug | 产品价格: | ¥3324.0 |
Research Areas
CancerUniprot ID
A4PB30Gene Names
apM1Alternative Name(s)
apM1Lead Time
3-7 business daysOrganism
Felis catus (Cat) (Felis silvestris catus)Source
E.coliExpression Region
18-244aaProtein Length
Full Length of Mature ProteinTag Info
Tag-FreeTarget Protein Sequence
QDSETEGPGVVVPLPKGACTGWMAGIPGHPGHNGTPGRDGRDGTPGEKGEKGDPGLVGPKGDTGETGVTGIEGPRGFPGIPGRKGEPGESAYVYRSAFSVGLESRVTVPNVPIRFTKIFYNQQNHYDVTTRKFHCNIPGLYYFSYHITVYLKDVKVSLYKRDKAMLFTYDQYQEKNVDQASGSVLLHLETGDEVWLQVYGDGDYNGLYADNVNDSTFTGFLLYYDTVMW
24.9 kDaPurity
Greater than 90% as determined by SDS-PAGE.Endotoxin
Not test.Biological_Activity
/Form
Liquid or Lyophilized powderBuffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.Storage
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.Relevance
/Function
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文献和实验卫星DNA 等遗传标记,先获得某一表型基因在染色体上的定位,再在候选区域内选择已知基因,进行致病突变的筛选,并获得cDNA 及全基因;另一类是从蛋白质功能着手的功能克隆 策略,采用以消减杂交为策略的多种分子生物学手段,先通过消减获得特异表达或缺失的基因片段,然后进行染色体定位乃至获得全基因。本文拟就前两种主要策略和各自方法的优缺点作一介绍和分析。此外,尚有介于两者之间的候选克隆 策略,包括定位候选克隆 和功能候选,前者是在将疾病基因以连锁分析和染色体分析基本定位以后,再在候选区域内选择所有已知基因进行致病突变
2细胞周期同步化和中期染色体富集 为了富集足量同步化的中期染色体,秋水仙碱常被用于抑制有丝分裂过程中纺缍丝的形成。但由于其对植物微管蛋白亲和力较低,需要使用较高的浓度,对人体会造成高毒性的危害以及在植物遗传上产生不稳定性。另外秋水仙碱也会导致染色体过于粘稠,不适用于染色体分拣等后续操作。1992年Dolezel等发现应用甲基氨草磷(APM’Apiprophos―methy1)、安磺灵(Oryzalin)、氟乐灵(Trifluralin)等人工除草剂代替秋水仙碱,在微摩级浓度时便可见显著的抗微管
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