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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Onchocerca volvulus OV-16 antigen (OV16)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
1mg/100μg/20μg
| 规格: | 1mg | 产品价格: | ¥17754.0 |
|---|---|---|---|
| 规格: | 100μg | 产品价格: | ¥4956.0 |
| 规格: | 20μg | 产品价格: | ¥2616.0 |
纯度:
Greater than 90% as determined by SDS-PAGE.基因名:
OV16Uniprot No.:
P31729别名:
OV16; OV-16 antigen种属:
Onchocerca volvulus蛋白长度:
Full Length of Mature Protein来源:
Yeast分子量:
22 kDa表达区域:
17-197aa氨基酸序列:
KISAENANCKKCTPMLVDSAFKEHGIVPDVVSTAPTKLVNVSYNNLTVNLGNELTPTQVKNQPTKVSWDAEPGALYTLVMTDPDAPSRKNPVFREWHHWLIINISGQNVSSGTVLSDYIGSGPRKGTGLHRYVFLVYKQPGSITDTQHGGNRRNFKVMDFANKHHLGNPVAGNFFQAKHED蛋白标签:
N-terminal 6xHis-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.货期:
25-35 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能:
/内毒素:
Not test.SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.LC-MS Image Description/Western Blot:
/Product types:
Developed ProteinBiological_Activity:
/Research Areas:
OthersReconstitution:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.Reference:
Identification of an Onchocerca volvulus cDNA encoding a low-molecular-weight antigen uniquely recognized by onchocerciasis patient sera.Lobos E., Altmann M., Mengod G., Weiss N., Rudin W., Karam M.Mol. Biochem. Parasitol. 39:135-146(1990)Function:
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文献和实验, X, et al. A Fluorescent Microbead-based Microfluidic Immunoassay Chip for Immune Cell Cytokine Secretion Quantification. Lab Chip 18(3):522-531, 2018. [26] Huis In 't Veld LG, et al. Saponin-based adjuvant uptake and induction of antigen cross
3、峰面积百分率法以色谱中所得各种成分的峰面积的总和为100,按各成分的峰面积总和之比,求出各成分的组成比率。气液色谱法这时所指的气液色谱法,主要用于各种香料物质的分析,基本条件和参数主要依照美国精油协会(EOA)于1979年所建议的方法。其基本原理、操作、标准状态等均与上述气相色谱法相同。1、柱用304号合金所制不锈钢管,长3m,内径2.16-2.57mm,外径3.18mm。底物:极性柱为聚乙二醇20M(Carbowax20M),分子量约2万;非极性柱为气相色谱级甲基硅氧烷
检查(包括进样垫),确保整个流路系统不漏气。 (6)、气源压力过低(如不足10~15个大气压),气体流量不稳,应及时更换新钢瓶,保持气源压力充足、稳定。 (7)、对新填充的色谱柱,一定要老化充分,避免固定液流失,产生噪音。以OV-101、OV-17、OV-225等试剂级固定液,老化时间不应该少于24小时,对SE-30,QF-1工业级的固定液因纯度低,老化不应该少于48小时。 (8)、注射器要经常用溶剂(如丙酮)清洗。试验结束后,立即清洗干净,以免被样品中的高沸点物质污染
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