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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Chicken Avidin (AVD)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Chicken Avidin (AVD)品名简称:
Recombinant Chicken AVD protein货号:
CSB-YP355865CH规格:
20μg价格:
2616LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 85% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
AVDUniprot No.:
P02701别名:
/种属:
Gallus gallus (Chicken)蛋白长度:
Full Length of Mature Protein来源:
Yeast分子量:
15.8 kDa表达区域:
25-152aa氨基酸序列:
ARKCSLTGKWTNDLGSNMTIGAVNSRGEFTGTYITAVTATSNEIKESPLHGTQNTINKRTQPTFGFTVNWKFSESTTVFTGQCFIDRNGKEVLKTMWLLRSSVNDIGDDWKATRVGINIFTRLRTQKE蛋白标签:
C-terminal 6xHis-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
25-35 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
The biological function of avidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of avidin).功能2:
/文献:
"Biochemical characterization and crystal structure of a recombinant hen avidin and its acidic mutant expressed in Escherichia coli." Nardone E., Rosano C., Santambrogio P., Curnis F., Corti A., Magni F., Siccardi A.G., Paganelli G., Losso R., Apreda B., Bolognesi M., Sidoli A., Arosio P. Eur. J. Biochem. 256:453-460(1998)研究领域:
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文献和实验存在于卵白中的碱性糖蛋白,因它贪婪地(avidly)与生物素结合,故有此名( avidin)。给动物以生的卵白则产生生物素缺乏症,因为生物素蛋白与生物素结合,使之失活。分子量为 68300,含 4个亚单位。在体外(试管中)对含生物素的酶具有特异的抑制作用。
Links Gallus gallus lipoprotein lipase (LPL), mRNA gi|49258193|ref|NM_205282.1|[49258193] 607:Chicken mRNA for adipose lipoprotein lipase (EC 3.1.1.34) gi|63573|emb|X14670.1|GGLPL[63573] 608: X60547 Reports Links Chicken lipoprotein lipase gene
生物素亲和素(又称抗生物素)系统(biotin avidin system,BAS)标记抗体的技术是 20 世纪 70 年代后期发展起来的一种新的免疫检测方法。 由于亲和素与生物素间的亲和力极强,结合迅速,且极其稳定,生物素标记抗体和酶的标记率高,又不影响蛋白的活性,使 BAS 标记技术比常规酶联免疫、放射免疫及荧光免疫技术有着更高的灵敏度,为微量抗原、抗体的检测开辟了新的途径。近年来,在 BAS 检测中多用链霉亲和素“streptavidin,SA”,它是链霉菌培养过程中的分泌物,完整的链霉
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