- ￥5600
- GeneTex
- 美国
- GTX25487
- 2025年07月14日
- WB, FACS, ChIP assay
- Rabbit
- Human, Mouse, Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human Smad2 that contains threonine 8. The sequence is conserved in mouse and rat.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Rat
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX25487
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, FACS, ChIP assay
- 浓度：
Batch dependent (Please refer to the vial label for the specific concentration.)
- 靶点：
SMAD2 (phospho Thr8)
- 抗体英文名：
SMAD2 (phospho Thr8) antibody
- 抗体名：
SMAD2 (phospho Thr8) 抗体
- 规格：
50 μl
ChIP analysis of 2 million Jurkat cells treated with 50ng/ml of TGF-beta for one hour using GTX25487 SMAD2 (phospho Thr8) antibody. Normal Rabbit IgG was used as a negative IP control. The precipitated DNA was detected by PCR with primer set targeting to the promoter of ID1 gene as positive control and the inactive SAT2 used as negative control target.
WB (peptide competition) analysis of HepG2 cells stimulated with TGF beta using GTX25487 SMAD2 (phospho Thr8) antibody prior incubated with the non-phosphopeptide corresponding to the immunogen (Lane 2), a generic phosphothreonine containing peptide (Lane 3), or, the phosphopeptide immunogen (Lane 4) control. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody. The membrane treated with phosphatase (Lane 5) eliminates the signal further verifying that the antibody is phospho-specific.
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文献和实验Optimized Protocol to Make Phospho-Specific Antibodies that Work
, not simply its level of expression. In this review, we will discuss both the design of the phosphopeptide immunogen and immunization. The affinity purification of the phospho-specific antibody as well as the methods most suitable for characterizing
Using Phospho‐Motif Antibodies to Determine Kinase Substrates
comprising both the phosphorylated residue and the surrounding residues that determine kinase specificity, with degenerate residues taking up the remaining positions. Currently, several categories of phospho?motif antibody are commercially available
Absorption Control in Immunohistochemistry Using Phospho-Peptides Immobilized on Magnetic Beads
neutralization of phospho-specific antibodies with phospho-peptides immobilized on magnetic beads. This technique allows for sequestration of antibody–peptide complex from the incubation solution, minimizing the risk of formation of unblocked antibodies capable
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