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- BioToolomics
- 英国
- 360201-5ML
- 2025年07月12日
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- 文献和实验
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- 库存：
大量
- 供应商：
北京百奥创新科技有限公司
- 规格：
5mL;50mL;1L
肼活化磁性琼脂糖凝胶4HF
产品名称：肼活化磁性琼脂糖凝胶4HF英文名称：Hydrazide-activated SepFast MAG 4HF
Product Introduction:
Hydrazide activated SepFast MAG is a magnetic agarose resin that is particularly useful for making glycoprotein-attached magnetic adsorbents.
Hydrazide-activated agarose permits the coupling of aldehyde- or ketone-containing ligands through the formation of stable hydrazone linkages. It is a particularly powerful way to immobilize glycoproteins via their carbohydrate chains while leaving critical active sites intact. It is a method called “site-directed immobilization”. For example, antibodies can be immobilized to an agarose solid support through a linkage to their glycosylated side chain that leaves their Fab region free for antigen binding.
Glycoproteins can be readily oxidized with sodium periodate to generate formyl groups on their carbohydrate chains before the coupling reaction with Hydrazide-activated SepFast MAG. It is a successful and well-documented technique. The coupling reaction is mild and easy to carry out. No toxic chemical or special equipment is required.
Hydrazide-activated SepFast MAG resins can be readily employed to make custom affinity chromatography media for both small scale and large scale purification applications.
Product Properties:
Hydrazide activated SepFast MAG 4HF is made of highly cross-linked 4% agarose containing magnetic material. It shows high mechanical rigidity and high chemical stability for harsher operational conditions.
Agarose has long been used for chromatographic separations due to its excellent hydrophilic and low non-specific-binding nature. The particles have an open pore structure with excellent mass transfer properties to large protein molecules.
The base matrix is activated with hydrazide through a long hydrophilic spacer arm. Hydrazideactivated SepFast MAG media is supplied as an aqueous suspension.
Product Series:
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文献和实验S.L. Lim, H.W. Ng, M.A. Akwiditya, C.W. Ooi, E.S. Chan, K.L. Ho, W.S. Tan, G.K. Chua, B.T. Tey, Single-step purification of recombinant hepatitis B core antigen Y132A dimer from clarified Escherichia coli feedstock using a packed bed anion exchange chromatography, Process Biochemistry, 2018, 69:208-215.
酯、酰氯或用强的失去剂（如碳二亚氨）形成对称酸酐等方法来形成酰胺键。其中选用DCC、HOBT或HOBT/DCC的对称酸酐法、活化酯法接肽应用最广。 裂解及合成肽链的纯化 BOC法用TFA+HF裂解和脱侧链保护基，FMOC法直接用TFA，有时根据条件不同，其它碱、光解、氟离子和氢解等脱保护方法也被采用。合成肽链进一步的精制、分离与纯化通常采用高效液相色谱、亲和层析、毛细管电泳等。 HPLC分析和纯化 分析HPLC使用柱子和泵系统，可以经受传递高压，这样可以用极细的微粒（3-10μ m）做填料
(Fmoc:9-芴甲氧羰基).以这两种方法为基础的各种肽自动合成仪也相继出现和发展，并仍在不断得到改造和完善. Merrifield所建立的Boc合成法［2］是采用TFA(三氟乙酸)可脱除的Boc为α-氨基保护基，侧链保护采用苄醇类.合成时将一个Boc－氨基酸衍生物共价交联到树脂上，用TFA脱除Boc，用三乙胺中和游离的氨基末端，然后通过Dcc活化、耦联下一个氨基酸，最终脱保护多采用HF法或TFMSA(三氟甲磺酸)法.用Boc法已成功地合成了许多生物大分子，如活性酶、生长因子、人工蛋白等.
保护基，侧链保护采用苄醇类.合成时将一个Boc－氨基酸衍生物共价交联到树脂上，用TFA脱除Boc，用三乙胺中和游离的氨基末端，然后通过Dcc活化、耦联下一个氨基酸，最终脱保护多采用HF法或TFMSA(三氟甲磺酸)法.用Boc法已成功地合成了许多生物大分子，如活性酶、生长因子、人工蛋白等. 多肽是涉及生物体内各种细胞功能的生物活性物质。它是分子结构介于氨基酸和蛋白质之间的一类化合物,由多种氨基酸按照一定的排列顺序通过肽键结合而成。到现在，人们已发现和分离出一百多种存在于人体的肽，对于多肽的研究和利用，出现
