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- BioToolomics
- 英国
- 390101-50ML
- 2025年07月15日
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- 文献和实验
- 技术资料
- 库存：
大量
- 供应商：
北京百奥创新科技有限公司
- 现货状态：
现货
- 保修期：
详询
- 规格：
5mL;50mL;1L
不可逆硫醇耦联琼脂糖介质4HF
产品名称：不可逆硫醇耦联琼脂糖介质4HF
英文名称：Irreversible Thiol-coupling SepFast
Product Introduction:
Irreversible Thiol-coupling SepFast can be used for the specific immobilization of thiol containing molecules. The coupling chemistry is based on a haloacetyl activation method that is a successful and well-documented technique. The reaction to the thiol group is highly specific and the formed bond is irreversible. The coupling reaction is rapid and easy to carry out. No toxic chemicals or equipment is required.
Irreversible Thiol-coupling SepFast media forms an extremely stable thioether bond with ligands containing thiol (i.e. sulphhydryl) groups. It has a long spacer arm that facilitates much improved ligand accessibility. This pre-activated agarose based resin can be readily employed to make various custom affinity chromatography media for both small scale and large scale purification applications.
Product Properties:
There is a choice of two different activated base matrices that suit ligands of various molecular weights.
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Irreversible Thiol-Coupling SepFast 4HF is made of highly cross-linked 4% beaded agarose. It shows high mechanical rigidity allowing high flow throughput with reduced back pressure in a packed column.
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Irreversible Thiol-Coupling SepFast 6HF is made of highly cross-linked 6% beaded agarose. It shows high mechanical rigidity allowing high flow throughput with reduced back pressure in a packed column.
Agarose has long been used for chromatographic separations due to its excellent hydrophilic and low non-specific-binding nature. The particles have an open pore structure with excellent mass transfer properties to various molecules.
The base matrix is activated by attaching a haloacetyl group through a long hydrophilic spacer arm. It reacts rapidly with molecules containing thiol groups. Irreversible Thiol-coupling SepFast media is supplied as an aqueous suspension.
Product Series:
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文献和实验M.F.X. Lee, E.S. Chan, W.S. Tan, K.C. Tam, B.T. Tey, Negative chromatography of hepatitis B virus-like particle: Comparative study of different adsorbent designs, Journal of Chromatography A, 2016, 1445:1-9.
S.L. Lim, H.W. Ng, M.A. Akwiditya, C.W. Ooi, E.S. Chan, K.L. Ho, W.S. Tan, G.K. Chua, B.T. Tey, Single-step purification of recombinant hepatitis B core antigen Y132A dimer from clarified Escherichia coli feedstock using a packed bed anion exchange chromatography, Process Biochemistry, 2018, 69:208-215.
，通过化学方法引入电荷基团制成的。 1、按引入电荷基团的性质分类： （1）阳离子交换琼脂糖：CM－Sepharose CL－6B 等。 （2）阴离子交换琼脂糖：DEAE－Sepharose CL－6B 等。 2、特点： 具有硬度大、性质稳定、网孔大、流速好和分离能力强等特点，尤其是介质对 pH 和温度的变化均比较稳定，可在 pH = 3～10 和 0～70℃ 范围内使用，改变离子强度或 pH 时，床体积变化不大。 3、应用： 特别适合分离相对分子质量大的蛋白
网状结构。以使被分离的生物分子能够均匀、稳定的通透，并充分与配体结合。基质的孔径过小会增加基质的排阻效应，使被分离物与配体结合的机率下降，降低亲和层析的吸附容量。所以一般来说，多选择较大孔径的基质，以使待分离物有充分的空间与配体结合。4.基质本身与样品中的各个组分均没有明显的非特异性吸附，不影响配体与待分离物的结合。基质应具有较好的亲水性，以使生物分子易于靠近并与配体作用。一般纤维素以及交联葡聚糖、琼脂糖、聚丙烯酰胺、多孔玻璃珠等用于凝胶排阻层析的凝胶都可以作为亲和层析的基质，其中以琼脂糖
极性固定相间的相互作用力（非选择性分散力或伦敦力）大小与溶质分子极性基团与流动力相中极性分子在相反方向上相互作用力的差异进行分离。因其流动相中的置换剂是极性小于水的有机溶剂（如甲醇、乙腈、四氢呋喃等），这些有机溶剂可能使许多蛋白质分子产生不可逆的变性；流动相中须有离子对试剂（如三氟乙酸、甲酸、磷酸等）存在才可使分离有效地进行和获得高的质量回收率；分离须在酸性介质中进行（一般pH在2~3之间），又有一些蛋白质会在后两种条件下产生不可逆的分子构象变化，故在生物大分子中人分离纯化中受到限制，但分子
