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- 文献和实验
- 技术资料
- 英文名:
hepa1-6-luc
- 库存:
现货库存
- 供应商:
南京万木春
- 肿瘤类型:
/
- 细胞类型:
/
- 品系:
/
- 组织来源:
hepa1-6-luc
- 相关疾病:
/
- 物种来源:
/
- 免疫类型:
/
- 细胞形态:
/
- 是否是肿瘤细胞:
/
- 器官来源:
hepa1-6-luc
- 运输方式:
常温/干冰
- 年限:
/
- 生长状态:
/
- 规格:
T25
小鼠肝癌细胞带荧光素酶Hepa 1-6+LUC
| 种属 | 小鼠 |
| 别称 | Hepa 1-6+LUC |
| 组织来源 | 肝脏 |
| 疾病 | 小鼠肝细胞癌 |
| 传代比例/细胞消化 | 1:2传代 ,消化2-3分钟 |
| 完全培养基配置 | DMEM培养基;10%胎牛血清;1%双抗 |
| 简介 | 此细胞株源自C57/L小鼠中引发的BW7756肝癌;表达AFP、Α1抗胰蛋白酶、淀粉酶;鼠痘病毒阴性。此细胞可以在 无血清的培养基中繁殖 ,培养基成分是:DMEM,75%; WAYMOUTH’S MAB 87/3培养基 ,25%。添加3X10-8M 硒。 |
| 形态 | 上皮细胞样 |
| 生长特征 | 贴壁生长 |
| 倍增时间 | ~24-30h |
| 基因表达 | albumin, alpha fetoprotein (AFP, alpha-fetoprotein); albumin; alpha 1 antitrypsin (alpha-1-antitrypsin); amylase |
| 培养条件 | 气相:空气 ,95% ;二氧化碳 ,5%。 温度:37摄氏度 ,培养箱湿度为70%-80%。 |
| 冻存条件 | 冻存液:90%FBS ,DMSO 10%, 或使用非程序冻存液:官网货号JY-H040 |
| 保藏机构 | 该细胞是通过慢病毒转染荧光素酶的稳转株 ,收到细胞传代8代左右后 ,若要求需要维持荧光强度 ,建议可以加入嘌呤 霉素进行再次筛选。 |
| 产品使用 | 仅限于科学研究 ,不可作为动物或人类疾病的治疗产品使用。 |
In the past few years, cancer immunotherapies have produced promising results. Although traditionally considered unresponsive to immune therapy, increasing evidence indicates that ovarian cancers are, in fact, immunogenic tumors. This evidence comes from diverse epidemiologic and clinical data comprising evidence of spontaneous
Results: The cohort had a mean age of 39 years and included patients with severe polytrauma. Technical success was achieved in 95% of cases, with no intraoperative deaths or need for open surgical conversion. Perioperative complications were minimal, and the reintervention rate was 5.3%. This study evaluates 10 years of clinical experience managing TAI with TEVAR. Long-term follow-up, with a median duration of 29 months, revealed no graft-related complications or secondary interventions. Imaging confirmed sustained graft integrity, and clinical outcomes were favorable.
Conclusion: TEVAR is a reliable and effective treatment for traumatic aortic injuries, offering excellent technical success rates and minimal perioperative and long-term complications. This study highlights TEVARs advantages in managing polytrauma patients and its role as a minimally invasive alternative to open surgery. Additionally, the findings emphasize the need for comprehensive long-term follow-up protocols. Future research should aim to address challenges related to device durability and the integration of advanced imaging techniques to further enhance patient outcomes and broaden the applicability of TEVAR in trauma care.
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文献和实验In the past few years, cancer immunotherapies have produced promising results. Although traditionally considered unresponsive to immune therapy, increasing evidence indicates that ovarian cancers are, in fact, immunogenic tumors. This evidence comes from diverse epidemiologic and clinical data comprising evidence of spontaneous
越高。 此外,为了使 BiNR 探针能够运用于临床样本,研究者还优化了检测方法,可以在不需要转染荧光素酶基因的情况下监测 NR 通量,使 BiNR 探针可以应用于临床样本。 图 2:来源 Biosensors and Bioelectronics 烟酰胺核糖在癌症发展和扩散中的作用 该研究的通讯作者 Elena Goun 教授的父亲在被诊断出结肠癌后仅三个月就去世了。自此,Goun 以寻求对癌症代谢或癌症在体内扩散的能量的更好科学理解。由于 NR 是一种已知的补充剂,可以帮助提高细胞能量水平,而癌细胞通过增加
2)。 图2 使用BLOCK-iT™ Complete Dicer RNAi Kit得到>80%的靶定基因阻断 经Dicer酶切的,来源于β-半乳糖苷酶或者荧光素酶的dsRNA产生siRNA(d-siRNA)库,用来确定d-siRNA特异阻断基因活性的效果。GripTite™ 293 MSR 细胞共转染pcDNA™1.2/V5-GW/lacZ阳性对照质粒和pcDNA™5/FRT/luc,分别独自表达β-gal或者荧光素酶报告子,或者50ng luc 或者lacZ d-siRNA。对于每一种报告子
动物组织肿瘤细胞 AtT-20 小鼠垂体瘤 BC3H1 鼠脑瘤 BV-2 小鼠小胶质瘤细胞 C6 大鼠脑胶质瘤 Cyc-tAg 小鼠T淋巴细胞瘤(SV40T抗原转染) DCS 小鼠树突状细胞肉瘤(B类) EL-4 鼠T淋巴细胞瘤 FOX-NY 小鼠淋巴瘤细胞 GH3 大鼠垂体瘤细胞 HEPA1-6 小鼠肝癌细胞 MEL 小鼠红白血病细胞 MFC 小鼠前胃癌细胞 MMQ 大鼠垂体瘤细胞 NG108-15 小鼠神经细胞
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