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- 详细信息
- 文献和实验
- 技术资料
- 库存:
现货
- 英文名:
Recombinant Human TGF-alpha Protein
- 保质期:
一年
- 供应商:
翌圣生物科技(上海)股份有限公司
- 保存条件:
-20度
- 规格:
100ug
Product Properties
| Synonyms | Transforming Growth Factor alpha; TGF-a; TGF-type I; ETGF |
| Accession | P01135 |
| Gene ID | 7039 |
| Source | E.coil-derived Human TGF-a protein, Vla40-Ala89 |
| Molecular Weight | Approximately 6 kDa. |
| AA Sequence | V VSHFNDCPDS HTQFCFHGTC RFLVQEDKPA CVCHSGYVGA RCEHADLLA |
| Tag | None |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Purity | > 90% as determined by SDS-PAGE. |
| Biological Activity | Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 0.1-0.4 ng/mL. |
| Endotoxin | < 1.0 EU per 1μg of the protein by the LAL method. |
| Formulation | Lyophilized from 0.2 µm filtered concentrated solution in Acetonitrile and TFA. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 10 mM Acetic Acid to a concentration of 0.1 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions. Do not reconstitute in cell culture media directly. |
Shipping and StorageThe products are shipped with ice pack and can be stored at -20℃ for 1 year.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 °C under sterile conditions after reconstitution.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
Cautions
- For your safety and health, please wear lab coats and disposable gloves for operation.
- For research use only!
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文献和实验Analysis of TGF-Inducible Apoptosis
Transforming growth factor-β (TGF-β) is an important regulator of cellular growth and immune homeostasis (1 –3 ). TGFβ is a homodimeric 25-kDa protein which activates cellular signaling through the recruitment and transphos-phorylation
Radioiodinated transforming growth factors ( TGF-βs) are critical reagents for functional studies on TGF-β ligand-receptor binding (1 –5 ), TGF-β-binding protein interactions (6 –9 ), ligand uptake, degradation and clearance (1 ,10 ,11
Measuring Interactions Between ECM and TGF-Like Proteins
of growth factors in a readily mobilized form, which could allow extracellular signaling to proceed rapidly in the absence of new protein synthesis. This may be particularly important in situations such as tissue repair following injury. Storage of growth










