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- Serumwerk Bernburg AG
- 德国
- 18003
- 2025年07月15日
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- 保存条件：
常温
- 库存：
999
- 规格：
500g
Nycodenz
A universal density gradient medium
It was originally developed as an X-ray contrast medium and have therefore been subjected to rigorous clinical testing.
- non-ionic, non-toxic to cells and metabolically inert
- can be used for the isolation of cells, subcellular organelles and membranes, macromolecules and viruses
- forms true solutions. It is therefore easy to remove the medium from the cells after fractionation
- is resistant to bacterial degradation
- solutions can be autoclaved
Nycodenz is the trademark name for iohexol, whose systematic name is 5-(N-2,3-dihydroxypropylacetamido)-2,4,6-tri-iodo-N-N’-bis(2,3-dihydroxypropyl)isophthalamide. It has a molecular weight of 821 g/mol. The chemical properties stability of Nycodenz are related to its structure.
Gradients of Nycodenz can be generated by:
- Centrifugation in situ (self-forming gradients).
- Diffusion. Using Nycodenz, linear gradients can be simply prepared within 45 minutes.
- Freezing and thawing.
- Tilted tube rotation (Gradient Master™).
The density of Nycodenz in solution can be determined by measuring the refractive index. The density can also be determined spectrophotometrically.
Nycodenz is a non-particulate medium therefore the distribution of cells in gradients can be determined using a haemocytometer, electronic particle counter or by light scattering measurements using a spectrophotometer.
Nycodenz does not interfere with the orcinol and diphenylamine reactions for the estimation of nucleic acids nor with the very sensitive dye binding assays for protein and DNA.
Fluorimetric assays of nucleic acids and proteins can also be carried out in the presence of Nycodenz. Nycodenz does not interfere with most assays for the marker enzymes of subcellular components, also commercial scintillants are compatible with Nycodenz.
Nycodenz can be removed from samples by dialysis, ultrafiltration or gel filtration. Cells, subcellular organelles and other particulate matter can be isolated from Nycodenz by centrifugation without the risk of contaminating the pellet with Nycodenz.
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文献和实验Isolation and Culture of Hepatic Stellate Cells
Hepatic stellate cells (HSCs) are routinely prepared by collagenase/pronase digestion of liver using a perfusion system and subsequent fractionation of the heterogenous cell suspension on continuous density gradients made out of Nycodenz
Isolation of Mitochondria From Procyclic Trypanosoma brucei
occurs isotonically by N2 cavitation, and the mitochondrial vesicles are isolated by Nycodenz gradient centrifugation.
材料相差显微镜，荧光显微镜，手术器械，雄性SD大鼠，体重250-450 g，戊巴比妥钠，200目尼龙网，小牛血清（或胎牛血清，则更好），DMEM，胰酶消化液（以HBSS配），Nycodenz（以GBSS配），D-Hanks'（KCl 0.4 g/L，KH2PO4 0.06 g/L，NaCl 8.0 g/L，NaHCO3 0.35 g/L，Na2HPO4.7H2O 0.06 g/L或Na2HPO4 0.053 g/L，可加酚红 0.02 g/L），HBSS，台盼兰等。方法大鼠腹腔麻醉-->剖腹
