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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
A synthetic peptide of human Histone H2A.X
- 亚型:
IgG
- 保存条件:
Store at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
- 克隆性:
Monoclonal
- 适应物种:
Human, Mouse, Rat
- 库存:
99
- 宿主:
Rabbit
- 应用范围:
WB, IHC, ICC/IF, FC, IP
- 靶点:
Histone H2A.X
- 规格:
10μl
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文献和实验Competing Chromosomal Proteins from Drosophila Polytene Chromosomes Using Modified Histone Peptides
al ., 1996). In this adaptation, I used differently methylated histone tail peptides to compete Polycomb protein from polytene chromosomes, finding that competition was peptide specific and locus-specific (Ringrose et al ., 2004). (I also tried RNAse
INTRODUCTION In cells and tissues, the histone proteins that constitute the nucleosomes can present multiple post-translational modifications, such as lysine acetylation, lysine and arginine methylation, serine phosphorylation
TBS 和 5% NGS 中封闭样品。市售的含有酪蛋白的封闭溶液与磷酸化的一抗结合后易减弱信号;因此,我们建议不使用含有酪蛋白的封闭剂进行磷酸化特异性抗体的检测。以上针对封闭步骤所提建议均是在以 SignalStain. Boost IHC Detection Reagent 作为检测试剂的情况下提出的。如果选择使用其他检测试剂,我们则建议使用与二抗来源相同的血清进行封闭。抗体:Phospho–Histone H2A.X (Ser139) (20E3) Rabbit mAb #9718样本:石蜡
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