- ￥1500 - 2300
- 爱必信(absin)
- 上海
- abs148526
- 2025年07月11日
- WB 1:500 - 1:1000, IHC 1:50 - 1:100, IHC 1:50 - 1:200
- Rabbit
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Ki67. The exact sequence is proprietary.
- 亚型：
-
- 形态：
液态
- 保存条件：
Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
- 克隆性：
-
- 标记物：
-
- 适应物种：
Human
- 保质期：
Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles
- 抗原来源：
-
- 目录编号：
-
- 级别：
-
- 库存：
现货
- 供应商：
爱必信(上海)生物科技有限公司
- 宿主：
Rabbit
- 应用范围：
WB 1:500 - 1:1000, IHC 1:50 - 1:100, IHC 1:50 - 1:200
- 浓度：
-
- 靶点：
-
- 抗体英文名：
,Antigen KI-67
- 抗体名：
Ki67抗体
- 规格：
50ul/100ul
| 规格： | 50ul | 产品价格： | ￥1500.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2300.0 |
| 概述 | |
| 描述 | Rabbit polyclonal antibody to Ki67 |
| 别名 | Ki67抗体;Antigen KI-67 |
| 宿主 | Rabbit |
| 特异性 | Recognizes endogenous levels of Ki67 protein. |
| 反应种属 | Human |
| 应用 | WB 1:500 - 1:1000, IHC 1:50 - 1:100, IHC 1:50 - 1:200 |
| 分子量 | Detects a band of approximately 358kD Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| 免疫原 | KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Ki67. The exact sequence is proprietary. |
| 性能 | |
| 纯化方法 | The antibody was purified by immunogen affinity chromatography. |
| 类型 | Polyclonal Antibody |
| 储存/保存方法 | Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles. |
| 靶标 | |
| UniProt | P46013 |
实验结果图：
Western blot analysis of Ki67 expression in HCT116 (A), U87MG (B), HepG2 (C), LO2 (D), SKOVCAR3 (E) whole cell lysates.
Immunohistochemical analysis of Ki67 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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baibai17 今天开题，导师问了个问题：ki67为什么叫ki67？当场傻住······恳请各位老师指教！谢谢。 keavin521 The Ki-67 protein was originally defined by the prototype monoclonal antibody Ki-67 (Gerdes et al., 1983),which was generated by immunizing mice
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for 25 minutes. 3. Incubate the tissue section with blocking buffer for 20 minutes. 4. Incubate the tissue section overnight at 4?C with Rabbit Anti-Murine RELMα at 4.0 ng/mL in 1X PBS with 0.01% Triton-X and 0.5% BSA. Wash the slide
抗核抗体(anti-nucleic antibody，ANA)检测
抗核抗体(anti-nucleic antibody，ANA)是以真核细胞的核成分为靶抗原的自身抗体的总称。细胞核内含有许多不同成分如核蛋白、核糖核蛋白(ribonucleo protein，RNP)、脱氧核糖核酸(DNA)等。 在某些因素，如细菌、病毒、药物等作用下，可改变细胞核某些成分的性质，激发机体免疫系统产生许多抗不同核成分的抗体。
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