Rabbit Anti-Ki67 Polyclonal An

概述
描述	Rabbit polyclonal antibody to Ki67
别名	Ki67抗体;Antigen KI-67
宿主	Rabbit
特异性	Recognizes endogenous levels of Ki67 protein.
反应种属	Human
应用	WB 1:500 - 1:1000, IHC 1:50 - 1:100, IHC 1:50 - 1:200
分子量	Detects a band of approximately 358kD Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
免疫原	KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Ki67. The exact sequence is proprietary.
性能
纯化方法	The antibody was purified by immunogen affinity chromatography.
类型	Polyclonal Antibody
储存/保存方法	Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
靶标
UniProt	P46013

      实验结果图：


Western blot analysis of Ki67 expression in HCT116 (A), U87MG (B), HepG2 (C), LO2 (D), SKOVCAR3 (E) whole cell lysates.

Immunohistochemical analysis of Ki67 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.