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- 文献和实验
- 技术资料
- 保存条件:
零下80~20°C
- 保质期:
Powder: -20°C for 3 years In solvent: -80°C for 2 years
- 英文名:
Tunicamycin 14:1 Mixture
- 库存:
10
- 供应商:
TargetMol
- 规格:
500 μg/1 mg
| 规格: | 500 μg | 产品价格: | ¥8480.0 |
|---|---|---|---|
| 规格: | 1 mg | 产品价格: | ¥16100.0 |
Product Introduction
Bioactivity
英文名:Tunicamycin 14:1 Mixture
描述:Tunicamycin 14:1 is a mixture of tunicamycin structural isomers that contain a 14-carbon N-acyl chain with variable branching patterns. The N-acyl chain incorporated into tunicamycins, like tunicamycin 14:1, is derived from the same pool of cellular branched-chain fatty acids (BCFAs) in Streptomyces and directly impacts the biological activity of each individual tunicamycin variant.1,2,3Purified tunicamycin 14:1 with the iso branching configuration inhibits bacterial phospho-MurNAc-pentapeptide transferase (MraY) with an IC50 value of 0.31 μM.2

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文献和实验之mycoplasma (e.g. M. hyorhinis)。不需培养mycoplasma作为正反应对照组,避免可能之污染。 缺点︰PCR反应很灵敏,易有伪阳性结果。此方法尚在评估中,故结果仅作为参考和内部品管之一部份。 材料与设备: ATCC mycoplasma detection kit:可作50~100 reactions. 提供1st stage primer mixture与2nd stage primer mixture positive control DNA (A. laidlawii
.These are only rough guidelines. Detailed instructions about PCR laboratory setup and maintenance may be found in PCR Methods and Applications, 3, 2, S1-S14, 1993.Preparation of Reaction Mixture To perform several parallel reactions, we recommend the preparation
for a decrease of fluorescence lifetime. Total enhancement is the product of these two effects. The recent reviews (14 , 15 ) contain more details on MEF. 2. Model Immunoassays To demonstrate a metal-enhanced fluorescence immunoassay on the glass
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