- ￥3370 - 6380
- TargetMol
- 美国
- T38082
- 2025年07月14日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
零下80~20°C
- 保质期：
Powder: -20°C for 3 years In solvent: -80°C for 2 years
- 英文名：
Tunicamycin 15:1 Mixture
- 库存：
10
- 供应商：
TargetMol
- 规格：
500 μg/1 mg
| 规格： | 500 μg | 产品价格： | ￥3370.0 |
|---|---|---|---|
| 规格： | 1 mg | 产品价格： | ￥6380.0 |
Product Introduction
Bioactivity
英文名:Tunicamycin 15:1 Mixture
描述:Tunicamycin 15:1 is a mixture of tunicamycin structural isomers that contain a 15-carbon N-acyl chain with variable branching patterns. The N-acyl chain incorporated into tunicamycins, like tunicamycin 15:1, is derived from the same pool of cellular branched-chain fatty acids (BCFAs) inStreptomycesand directly impacts the biological activity of each individual tunicamycin variant.1,2,3Purified tunicamycin 15:1 withiso,anteiso, or a mixture ofisoandanteisobranching configurations inhibit bacterial phospho-MurNAc-pentapeptide transferase (MraY) with IC50values of 0.05, 0.36, and 0.09 μM, respectively.2
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文献和实验products (amplicons) that may reside in the laboratory environment.Guidance in Avoiding Contamination DNA sample preparation, reaction mixture assemblage and the PCR process, in addition to the subsequent reaction product analysis, should be performed
In Situ Hybridization Using Digoxigenin Labeled Probes
with DIG for Chromosome In Situ's (Ilaria Rebay, 1996) 2.0 ul 10X DIG Labeling Mixture (Boehringer Cat. #1277 065) 2.0 ul 10X Hexanucleotide Mixture (Boehringer Cat. #1277 081) 15.0 ul DNA + ddH2O 1.0 ul Klenow 20.0 ul Total Reaction
from described in our paper. 1) 50°C 2 min, 1 cycle 2) 95°C 10 min, 1 cycle 3) 95 °C 15 s -> 60 °C 30 s -> 72 °C 30 s, 40 cycles 4) 72°C 10 min, 1 cycle 3. A real-time PCR reaction mixture can be either 50 ml or 25 ml. Prepare the following
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