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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
A synthetic peptide around the H1047R region of PIK3CA, human origin.
- 亚型:
IgG
- 形态:
液体
- 保存条件:
零下20℃
- 克隆性:
多克隆
- 标记物:
FITC
- 适应物种:
脊椎动物
- 保质期:
24个月
- 抗原来源:
蛋白
- 目录编号:
TD-21146
- 级别:
科研级
- 库存:
999
- 供应商:
武汉天德
- 宿主:
兔
- 应用范围:
ELISA, WB, IHC,IP
- 浓度:
0.1 mg/ml
- 靶点:
H1047R
- 抗体英文名:
Anti-PIK3CA(H1047R) Rabbit polyclonal antibody
- 抗体名:
PIK3CA(H1047R) Polyclonal Antibody
- 规格:
100μl
PIK3CA(H1047R) Polyclonal Antibody
| Cat. #: TD-21146 |
| Size: 100 µL |
| Gene Symbol: PIK3CA(H1047R) |
| Description: Anti-PIK3CA(H1047R) Rabbit Polyclonal Antibody |
| Background: Phosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The catalytic subunit is encoded by the PIK3CA gene. It phosphorylates PtdIns, PtdIns4P and PtdIns(4,5)P2. The PIK3CA gene is one of the most frequently mutated genes in human cancers. |
| Immunogen: A synthetic peptide around the H1047R region of PIK3CA, human origin. |
| Applications: ELISA, WB, IHC,IP |
| Recommended Dilutions: ELISA: 1:100-1:5000 WB: 1:100-1:1000 IHC: 1:10-1:100 |
| Concentration: 0.1 mg/ml |
| Host Species: Rabbit |
| Format: Liquid |
| Clonality: Polyclonal |
| Isotype: IgG |
| Purity: Purified from serum |
| Preservative: No |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Anti-PIK3CA(H1047R) antibody recognizes PIK3CA(H1047R) of vertebrates. |
| Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing |
Western blot:
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文献和实验ELECTROPORATION OF ES CELLS AND ISOLATION OF H/R CLONES
4 2 x 106 EP + DNA Normal % survival after EP + DNA 5 2 x 106 EP + DNA G418 % total integrants ie random + H/R's
ELECTROPORATION OF ES CELLS AND ISOLATION OF H/R CLONES
5 2 x 106 EP + DNA G418 % total integrants ie random + H/R's 6 2 x 106 EP + DNA G418* Same as Plate 5 unless 7 2 x 106
xuchenmail 最近看了一篇文献,关于PIK3CA 基因突变的问题,文献说热突变位点在exon 9 (E542K, E545K) and exon 20 (H1047R),但是我根据pubmed上的数据,H1047R已经在21号外显子了,这是怎么回事啊?是文献错了吗?还是有其他的原因? zjubell 1.这篇文献是什么时候发表的?对序列更新也是很正常的事。另外,这篇文献IF是多少? 2.是否存在可变
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