Background: B-Raf is a member of the Raf family of Ser/Thr protein kinases. It functions downstream of Ras to regulate the MAP kinase signaling pathway. Mutations in the B-RAF gene cause diseases. Inherited mutations in B-RAF cause cardiofaciocutaneous syndrome. Acquired mutations in B-RAF have been found in cancers.
Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity: Recognizes B-Raf of vertebrates.
Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing
Immunohistochemistry:
Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue with anti- B-Raf polyclonal antibody (Cat. #TD-21271). Tissue samples were fixed with formaldehyde and blocked with 1% serum for 15 min at 37 °C. Antigen retrieval was by heat mediation in citrate buffer (pH6). Samples were then incubated with primary antibody (1:50 dilution) overnight at 4°C. A HRP-conjugated Goat anti-mouse IgG (1:50 dilution) was used as secondary antibody.