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- 详细信息
- 文献和实验
- 技术资料
- 库存:
大量
- 英文名:
HeLa Core Histones
- 保质期:
半年以上
- 供应商:
Active Motif
- 保存条件:
-80度
- 规格:
36 µg
人类子宫颈腺癌
提取物组成
HeLa核心组蛋白是通过羟基磷灰石柱色谱法从HeLa染色质中纯化出来的,在含有10 mM HEPES pH 7.9、1 mM EDTA、10 mM KCl、10%甘油、0.5 mM PMSF和1 mM DTT的储存缓冲液中配制。
质量控制
HeLa核心组蛋白在染色质组装试剂盒(目录号53500)中检测。
HeLa核心组蛋白通过SDS-PAGE检测。
储存
为确保稳定性,提取物应储存在-80℃。
我们建议将提取液等分到一次性使用的馏分中,然后储存在-80℃。这样可以避免重复冻融循环。
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文献和实验Isolation of Histones and Nucleosome Cores from Mammalian Cells
is often important for understanding mechanisms of regulation of transcription and other processes that occur on DNA. The basic unit of chromatin is the nucleosome core, containing two copies each of the core histones H2A, H2B, H3, and H4 to form a histone octamer
Expression and Purification of Recombinant Histones and Nucleosome Reconstitution
In vitro studies on nucleosome core particles (NCPs) and nucleosomes have generally been limited to the use of histone proteins isolated from chromatin. Numerous reliable and well-established methods have been described of obtaining single
Imaging of Vaccinia Virus Entry into HeLa Cells
. The methods presented here describe how to construct a fluorescent recombinant vaccinia virus expressing the core protein A4L fused to mCherry, and how to detect the virus movement on actin-EYFP-expressed HeLa cells.
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