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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
None
- 亚型:
IgG1, kappa
- 保存条件:
Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性:
Monoclonal
- 标记物:
FITC Plus Fluorescent Dye
- 适应物种:
n/a
- 保质期:
详见产品包装
- 库存:
充足
- 宿主:
Mouse
- 应用范围:
FC
- 靶点:
IgG1 Isotype Control
- 抗体英文名:
FITC Plus Mouse IgG1 Isotype Control (MOPC-21)
- 抗体名:
FITC Plus Mouse IgG1 Isotype Control (MOPC-21)
- 规格:
25ug/100ug
| 规格: | 25ug | 产品价格: | ¥600.0 |
|---|---|---|---|
| 规格: | 100ug | 产品价格: | ¥1200.0 |
经过测试的应用
| Positive FC detected in |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) | FC : 0.2 ug per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
FITC-65124 targets IgG1 Isotype Control in FC applications and shows reactivity with n/a samples.
| 经测试应用 | FC |
| 文献引用应用 | FC |
| 经测试反应性 | n/a |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Mouse / IgG1, kappa |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | immunoglobulin heavy constant gamma 1 (G1m marker) |
| 别名 | Ighg1, MOPC-21, Igh-4, IgG1 |
| 基因名称 | IgG1 Isotype Control |
| Gene ID (NCBI) | 16017 |
| RRID | AB_2883790 |
| 偶联类型 | FITC Plus Fluorescent Dye |
| 最大激发/发射波长 | 495 nm / 524 nm |
| 形式 | Liquid |
| 纯化方式 | Affinity purification |
| UNIPROT ID | P01868 |
| 储存缓冲液 | PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3. |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
The MOPC-21 immunoglobulin is useful as an isotype-matched control. The MOPC-21 immunoglobulin has an unknown binding specificity and is used as an isotype control for mouse IgG1 antibodies. This antibody has been quality-tested for flow cytometry as negative control.
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文献和实验| Species | Application | Title |
|---|---|---|
| FC | Int Immunopharmacol Neutrophil PPIF exacerbates lung ischemia-reperfusion injury after lung transplantation by promoting calcium overload-induced neutrophil extracellular traps formationAuthors - Wensi Wu | |
ACS Nano Orally Antigen-Engineered Yeast Vaccine Elicits Robust Intestinal Mucosal ImmunityAuthors - Xuenian Chen |
Rapid Isotyping of Mouse Monoclonal Antibodies
for 5% of mouse antibodies) has limited practical value. In contrast, the heavy-chain isotype is very informative (see Table 1 ); protein A or protein G binding, euglobulin properties, complement binding, kinetics of digestion with papain or pepsin, clearance
接收范围。 一般来说,实验panel有几种荧光,就需要设置几个单阳管。 同型对照(Isotype Control),是指与使用的流式抗体具有相同种属来源、亚型、荧光标记物、使用剂量和浓度,但对目标靶点无特异性结合的抗体。同型对照通常用来消除抗体的非特异性结合造成的背景染色。 FMO 对照是 Fluorescence Minus One(荧光扣除一)的缩写。在多色实验中,可能会出现某些通道信号难以区分的情况,主要原因是其他某个荧光对该通道的干扰很大,不能使用未染色的空白对照或全部用同型对照来设门
Immunofluorescent Staining of Mouse and Rat Leukocytes
steps 6-7). We find that SAv-PE and SAv-BD Cy-Chrome™ are "brighter" than FITC conjugates and may provide even better discrimination of low-density antigens, especially in the presence of BD Fc Block™, for mouse cells. (Please see Staining Tip
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