- 询价
- Cell Signaling Technology
- 3601
- USA
- 2025年12月13日
- W, IP
- Rabbit
- H
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
Phospho-BLNK (Tyr96) Antibody
- 抗原：
synthetic phosphopeptide corresponding to residues around Tyr96 of human BLNK
- 应用范围：
W, IP
- 宿主：
Rabbit
- 供应商：
CST
- 库存：
大量
- 适应物种：
H
- 保质期：
详见说明书
- 级别：
详见MSDS文件
- 是否单克隆：
2
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IP=Immunoprecipitation
Reactivity Key: H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP | H | Endogenous | 68, 70 | Rabbit |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | Phospho-BLNK (Tyr96) Antibody detects endogenous levels of BLNK only when phosphorylated at tyrosine 96. |
| Source / Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Tyr96 of human BLNK. Antibodies are purified by protein A and peptide affinity chromatography. Western Blotting
Western blot analysis of SDS extracts from control or anti-human IgM treated (12 µg/ml for 2 minutes) Ramos cells using Phospho-BLNK (Tyr96) Antibody. |
| Background | BLNK (B cell linker protein), also known as SLP-65 or BASH, is an adaptor molecule that plays key roles in B cell activation and B cell antigen receptor (BCR) engagement. BLNK acts on the interface between BCR-associated Syk and the downstream signaling cascades (1,2). BLNK has multiple SH2 binding motifs (YXXP) at its amino-terminus and a SH2 domain at its carboxy-terminus. After BCR ligation, BLNK is phosphorylated by Syk at multiple YXXP motifs including Tyr72, Tyr84, Tyr96 and Tyr178 (1). These phosphorylated motifs provide docking sites for signaling molecules such as Btk, PLCgamma and Vav. These signaling molecules bind to BLNK through their SH2 domains and together activate downstream signaling pathways (3,4). Through its SH2 domain, BLNK can also interact with tyrosine-phosphorylated targets such as HPK1 to recruit it to the BCR complex for signaling (5). |
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验Using Phospho‐Motif Antibodies to Determine Kinase Substrates
comprising both the phosphorylated residue and the surrounding residues that determine kinase specificity, with degenerate residues taking up the remaining positions. Currently, several categories of phospho?motif antibody are commercially available
Optimized Protocol to Make Phospho-Specific Antibodies that Work
, not simply its level of expression. In this review, we will discuss both the design of the phosphopeptide immunogen and immunization. The affinity purification of the phospho-specific antibody as well as the methods most suitable for characterizing
R&D Systems 96孔高通量蛋白芯片（Proteme Profiler 96 ）试剂盒技术专辑
图四 R&D Systems Proteme Profiler 96实验结果分析图 使用R&D Systems Proteme Profiler 96 蛋白芯片96检测胰岛素的受体酪氨酸激酶磷酸化水平。 A、使用R&D Systems Proteme Profiler 96 Antibody Array (目录号：ARZ001)同时检测经胰岛素处理5分钟和未经处理的肝癌细胞系HepG2细胞中16种受体酪氨酸激酶的磷酸化水平。每个微孔中的点阵成像
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