- 询价
- Cell Signaling Technology
- 4334
- USA
- 2025年08月05日
- W, IF-IC, F
- Rabbit
- H,Mk
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
AML1 Antibody
- 抗原：
synthetic peptide corresponding to amino acids near the amino terminus of human AML1
- 应用范围：
W, IF-IC, F
- 宿主：
Rabbit
- 库存：
大量
- 级别：
详见MSDS文件
- 供应商：
CST
- 适应物种：
H,Mk
- 保质期：
详见说明书
- 是否单克隆：
2
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IF-IC=Immunofluorescence (Immunocytochemistry) F=Flow Cytometry
Reactivity Key: H=Human Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IF-IC F | H Mk | Endogenous | 55 | Rabbit |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | AML1 antibody detects endogenous levels of total AML1 protein and the AML1/ETO fusion protein. |
| Source / Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino acids near the amino terminus of human AML1. Antibodies are purified by protein A and peptide affinity chromatography. Western Blotting
Western blot analysis of extracts from Jurkat and Kasumi-1 cells, using AML1 Antibody. Flow Cytometry
Flow cytometric analysis of Jurkat cells, using AML1 Antibody (blue) compared to a nonspecific negative control antibody (red). IF-IC
Confocal immunofluorescent image of Jurkat cells labeled with AML1 Antibody (green). Mitochondria have been labeled with MitoTracker Red CMXRos. |
| Background | AML1 (also known as Runx1, CBFA2, and PEBP2αB) is a member of the core binding factor (CBF) family of transcription factors (1,2). It is required for normal development of all hematopoietic lineages (3-5). AML1 forms a heterodimeric DNA binding complex with its partner protein CBFβ and regulates the expression of cellular genes by binding to promoter and enhancer elements. AML1 is commonly translocated in hematopoietic cancers: chromosomal translocations include t(8;21) AML1-ETO, t(12;21) TEL-AML, and t(8;21) AML-M2 (6). Phosphorylation of AML1 on several potential serine and threonine sites, including Ser249, is thought to occur in an Erk-dependent manner (7,8).
|
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验Generation of Antibody Molecules Through Antibody Engineering
been overcome to a large extent using genetic-engineering techniques to produce chimeric mouse/human and completely human antibodies. Such an approach is particularly suitable because of the domain structure of the antibody molecule ( 2 ), where functional
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