IKKβ Antibody

2684:

Western Blotting

IKKβ Antibody detects endogenous levels of total IKKβ protein. The antibody does not cross-react with endogenous levels of IKKα or IKKγ.

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues at the carboxy terminus of human IKKβ. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western blot analysis of extracts from HeLa, NIH/3T3 and PC12 cells, using IKKbeta Antibody.

The NF-κB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IκB proteins (1-3). Most agents that activate NF-κB do so through a common pathway based on phosphorylation-induced, proteasome-mediated degradation of IκB (3-7). The key regulatory step in this pathway involves activation of a high molecular weight IκB kinase (IKK) complex whose catalysis is generally carried out by three tightly associated IKK subunits. IKKα and IKKβ serve as the catalytic subunits of the kinase and IKKγ serves as the regulatory subunit (8,9). Activation of IKK depends upon phosphorylation of Ser177 and Ser181 in the activation loop of IKKβ (Ser176 and Ser180 in IKKα), which causes conformational changes resulting in kinase activation (10-13).

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• Makino, K. et al. (2004) Upregulation of IKKalpha/IKKbeta by integrin-linked kinase is required for HER2/neu-induced NF-kappaB antiapoptotic pathway. Oncogene 23, 3883-3887. Applications: Western Blotting
• Gao, H. et al. (2004) Identification of beta-arrestin2 as a G protein-coupled receptor-stimulated regulator of NF-kappaB pathways. Mol. Cell 14, 303-317. Applications: Western Blotting
• Pallotta, M.T. et al. (2011) Nat Immunol 12, 870-8. Applications: Western Blotting

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• 2078 Phospho-IKKα (Ser176)/IKKβ (Ser177) (C84E11) Rabbit mAb
• 2697 Phospho-IKKα/β (Ser176/180) (16A6) Rabbit mAb
• 2694 Phospho-IKKα/β (Ser176/180) Antibody II
• 2370 IKKβ (2C8) Rabbit mAb
• 2678 IKKβ (L570) Antibody
• 2682 IKKα Antibody
• 2695 IKKγ (DA10-12) Mouse mAb
• 2905 IKKε (D20G4) Rabbit mAb
• 3033 Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb
• 3036 Phospho-NF-κB p65 (Ser536) (7F1) Mouse mAb
• 3039 Phospho-NF-κB p65 (Ser468) Antibody
• 4812 IκBα (44D4) Rabbit mAb
• 4954 RelB Antibody
• 7071 Phototope^® -HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
• 7074 Anti-rabbit IgG, HRP-linked Antibody
• 7720 Prestained Protein Marker, Broad Range (Premixed Format)
• 7727 Biotinylated Protein Ladder Detection Pack
• 7003 20X LumiGLO^® Reagent and 20X Peroxide

For Research Use Only. Not For Use In Diagnostic Procedures.