- 询价
- Cell Signaling Technology
- 2148
- USA
- 2025年08月19日
- W, IHC-P, IF-IC, F
- Rabbit
- H,M,R,Mk,Z,B
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
α/β-Tubulin Antibody
- 抗原：
synthetic peptide corresponding to the sequence of human α- and β-tubulin
- 应用范围：
W, IHC-P, IF-IC, F
- 宿主：
Rabbit
- 供应商：
CST
- 库存：
大量
- 保质期：
详见说明书
- 级别：
详见MSDS文件
- 适应物种：
H,M,R,Mk,Z,B
- 是否单克隆：
2
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IHC-P=Immunohistochemistry (Paraffin) IF-IC=Immunofluorescence (Immunocytochemistry) F=Flow Cytometry
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey Z=Zebrafish B=Bovine
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IHC-P IF-IC F | H M R Mk Z B | Endogenous | 55 | Rabbit |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | The α/β-Tubulin Antibody detects endogenous levels of both α- and β-tubulin total protein, and does cross-react with both recombinant α- and β-tubulin. |
| Source / Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human α- and β-tubulin. Antibodies are purified by protein A and peptide affinity chromatography. Western Blotting
Western blot analysis of extracts from HeLa, NIH/3T3, C6 and COS-7 cells, using α/β-Tubulin Antibody. Western Blotting
Western blot analysis of recombinant alpha-tubulin and beta-tubulin GST-fusion proteins, and extracts from NIH/3T3 and C6 cells, using α/β-Tubulin Antibody (left) and GST Antibody #2622 (right). IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using α/β-Tubulin Antibody. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, showing cytoplasmic localization, using α/β-Tubulin Antibody. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human MALToma using α/β-Tubulin Antibody. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using β-Tubulin (9F3) Rabbit mAb #2128 in the presence of control peptide (left) or β-Tubulin Blocking Peptide (right). Flow Cytometry
Flow cytometric analysis of C6 cells using α/β-Tubulin Antibody (blue) compared to a nonspecific negative control antibody (red). IF-IC
Confocal microscopic images of NIH/3T3 cells showing cytoskeletal stain with α/β-Tubulin Antibody (A) compared to an isotype control (B). IF-IC
Confocal immunofluorescent analysis of NIH-3T3 cells, using α/β-Tubulin Antibody (green) and Phospho-Histone H3 (Ser10) (6G3) Mouse mAb #9706 (red) showing different stages of the cell cycle. |
| Background | The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with α/β-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. γ-tubulin is required to nucleate polymerization of tubulin subunits to form microtubule polymers. Many cell movements are mediated by microtubule action, including the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis/mitosis, and nerve-cell axon migration. These movements result from competitive microtubule polymerization and depolymerization or through the actions of microtubule motor proteins (1). |
| Application References |
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验的表达量就很少，此时 β-Actin 就不再适用于这些样本的内参。 【Tubulin】微管蛋白，是细胞的一种骨架蛋白。Tubulin 分为 α、β、γ、δ、ε等多种 tubulin，其中 α-Tubulin 和 β-Tubulin 可以形成异源二聚体，是形成微管的最主要的两种 Tubulin。α-tubulin 和β-tubulin 分子量分别为 55kDa 和 50kDa，其实际检测条带均在 55kDa 左右。作为内参，β-tubulin 的蛋白水平通常不会发生改变，因此被广泛用于 WB 时上样
Generation of Antibody Molecules Through Antibody Engineering
been overcome to a large extent using genetic-engineering techniques to produce chimeric mouse/human and completely human antibodies. Such an approach is particularly suitable because of the domain structure of the antibody molecule ( 2 ), where functional
祝贺〖雅裕睿安〗成为Signalway Antibody(SAB)独家
ul； 货号21617，产品描述：Tubulin beta-III Antibody；规格 50 ul / 100 ul； 货号21612，产品描述：GAPDH Antibody；规格 50 ul / 100 ul； 货号21335，产品描述：β-Tubulin mouse mAb；规格 50 ul / 100 ul； 货号21336，产品描述：GAPDH mouse
技术资料暂无技术资料 索取技术资料
